Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota 55905, United States.
Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
ACS Biomater Sci Eng. 2023 Mar 13;9(3):1585-1597. doi: 10.1021/acsbiomaterials.2c01482. Epub 2023 Feb 28.
Current bone cement systems often demand free radical or metal-related initiators and/or catalysts for the crosslinking process, which may cause serious toxicity to the human body. In addition, the resultant dense scaffolds may have a prolonged degradation time and are difficult for cells to infiltrate and form new tissue. In this study, we developed a porous "click" organic-inorganic nanohybrid (PO-click-ON) cement that crosslinks via metal-free biorthogonal click chemistry and forms porous structures mimicking the native bone tissue via particulate leaching. Strain-promoted click reaction enables fast and efficient crosslinking of polymer chains with the exclusion of any toxic initiator or catalyst. The resulting PO-click-ON implants supported exceptional stem cell adhesion and osteogenic differentiation with a large portion of stem cells infiltrated deep into the scaffolds. study using a rat cranial defect model demonstrated that the PO-click-ON system achieved outstanding cell adsorption, neovascularization, and bone formation. The porous click cement developed in this study serves as a promising platform with multifunctionality for bone and other tissue engineering applications.
目前的骨水泥系统通常需要自由基或金属相关的引发剂和/或催化剂来进行交联反应，这可能会对人体造成严重的毒性。此外，所得的致密支架可能降解时间较长，细胞难以渗透并形成新的组织。在这项研究中，我们开发了一种多孔的“点击”有机-无机纳米杂化（PO-click-ON）水泥，它通过无金属双正交点击化学交联，并通过颗粒浸出形成模仿天然骨组织的多孔结构。应变促进的点击反应能够快速有效地交联聚合物链，而无需任何有毒的引发剂或催化剂。所得的 PO-click-ON 植入物支持出色的干细胞黏附和成骨分化，大部分干细胞渗透到支架的深部。使用大鼠颅缺损模型的研究表明，PO-click-ON 系统实现了出色的细胞吸附、新血管生成和骨形成。本研究开发的多孔点击水泥是一种具有多功能性的有前途的骨和其他组织工程应用平台。
