浙江大学生高度近视易感性与 RASGRF1 基因多态性的相关性。

Correlation between High Myopia Susceptibility and Polymorphisms of RASGRF1 Gene among College Students in Zhejiang.

机构信息

Optometry Technology of Zhejiang Industry and Trade Vocational College, Wenzhou 325000, Zhejiang, China.

出版信息

J Environ Public Health. 2023 Feb 21;2023:6767410. doi: 10.1155/2023/6767410. eCollection 2023.

DOI:10.1155/2023/6767410

PMID:36864897

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9974243/

Abstract

OBJECTIVE

The aim of the study is to analyze the correlation between high myopia susceptibility and Ras protein-specific guanine nucleotide-releasing factor-1(RASGRF1) gene polymorphism among college students in Zhejiang.

METHODS

A stratified whole-group sampling method was used to select 218 cases of college students in Zhejiang who met the inclusion and exclusion criteria from January, 2019, to December, 2021, and they were divided into 77 cases (154 eyes) in the high myopia group and 141 cases (282 eyes) in the medium-low myopia group according to the degree of myopia, and 109 cases of college volunteers without myopia from the same period of medical examination in the region were included in the control group. The single nucleotide polymorphisms (SNPs) located in functional regions were selected by searching the literature and genetic databases, and the base sequences of rs939658, rs4778879, and rs8033417 loci were obtained by genotyping candidate SNPs using multiplex ligase detection reaction technique. The cardinality test was used to compare the differences in genotype frequency distribution of each locus of the RASGRF1 gene between the high myopia group and the low to moderate myopia group and the control group.

RESULTS

The genotype frequencies and allele frequencies of the RASGRF1 gene rs939658 locus in the high myopia group compared with the moderate-low myopia group and the control group were not statistically significant ( > 0.05). The genotype frequencies and allele frequencies of the rs4778879 locus of the RASGRF1 gene were compared among the three groups, and the differences were not statistically significant ( > 0.05). The genotype frequency and allele frequency of the rs8033417 locus of the RASGRF1 gene differed significantly among the three groups ( < 0.05).

CONCLUSION

The polymorphism of the rs8033417 locus of the RASGRF1 gene was significantly correlated with the susceptibility of high myopia among college students in Zhejiang.

摘要

目的

本研究旨在分析浙江大学生高度近视易感性与 Ras 蛋白特异性鸟嘌呤核苷酸释放因子 1（RASGRF1）基因多态性的相关性。

方法

采用分层整群抽样方法，选取 2019 年 1 月至 2021 年 12 月符合纳入和排除标准的浙江大学生 218 例，根据近视程度分为高度近视组 77 例（154 眼）、中低度近视组 141 例（282 眼），同期选取该地区体检的无近视大学生志愿者 109 例纳入对照组。通过文献和遗传数据库搜索，选择位于功能区域的单核苷酸多态性（SNP），采用多重连接酶检测反应技术对候选 SNP 进行基因分型，获得 rs939658、rs4778879 和 rs8033417 位点的碱基序列。采用卡方检验比较 RASGRF1 基因各位点基因型频率分布在高度近视组与中低度近视组和对照组之间的差异。

结果

高度近视组与中低度近视组和对照组相比，RASGRF1 基因 rs939658 位点的基因型频率和等位基因频率差异无统计学意义（>0.05）。RASGRF1 基因 rs4778879 位点的基因型频率和等位基因频率在三组间比较，差异无统计学意义（>0.05）。RASGRF1 基因 rs8033417 位点的基因型频率和等位基因频率在三组间差异有统计学意义（<0.05）。