Zhao Peng-Yu, Yang Yue-Qin, Wang Fei-Fan, Peng Min, Li Ming-Cong, Pei Dong, Hou Zhi-Yang, Zhou Yu-Bi
College of Agriculture, Henan University of Science and Technology Luoyang 471000, China.
Northwest Institute of Plateau Biology, Chinese Academy of Sciences Xining 810008, China.
Zhongguo Zhong Yao Za Zhi. 2023 Feb;48(4):908-920. doi: 10.19540/j.cnki.cjcmm.20220918.104.
To clarify the content characteristics of the main active components and mineral elements of Cynomorium songaricum under different habitat conditions, and further explore the relationship between the quality of C. songaricum and habitats, this study took C. songaricum from 25 different habitats in China as the research object, and measured the contents of 8 main active components and 12 mineral elements separately. Diversity analysis, correlation analysis, principal component analysis and cluster analysis were carried out. The results showed that the genetic diversity of total flavonoids, ursolic acid, ether extract, potassium(K), phosphorus(P) and zinc(Zn) in C. songaricum was high. The coefficient of variation of crude polysaccharide, ether extract, gallic acid, protocatechuic aldehyde, catechin, epicatechin, calcium(Ca), sodium(Na), magnesium(Mg), sulfur(S), iron(Fe), manganese(Mn), selenium(Se) and nickel(Ni) were all over 36%, indicating that the quality of C. songaricum was significantly affected by habitats. There were strong synergistic and weak antagonistic effects among the contents of the 8 active components, and complex antagonistic and synergistic effects among the contents of the 12 mineral elements. Principal component analysis revealed that crude polysaccharide, ursolic acid, catechin, epicatechin and total flavonoids could be used as the characteristic components to evaluate the quality of C. songaricum, and Na, copper(Cu), Mn and Ni were the characteristic elements to evaluate the quality of C. songaricum. In cluster ana-lysis, the second group with the main active components as cluster center had better quality in terms of the content of active substances, and the second group with the mineral elements as cluster center had higher utilization potential in the exploitation of mineral elements. This study could provide a basis for resource evaluation and breeding of excellent varieties of C. songaricum in different habitats, and provide a reference for cultivation and identification of C. songaricum.
为阐明不同生境条件下锁阳主要活性成分和矿质元素的含量特征,进一步探究锁阳品质与生境的关系,本研究以采自中国25个不同生境的锁阳为研究对象,分别测定了8种主要活性成分和12种矿质元素的含量,并进行了多样性分析、相关性分析、主成分分析和聚类分析。结果表明,锁阳中总黄酮、熊果酸、乙醚提取物、钾(K)、磷(P)和锌(Zn)的遗传多样性较高。粗多糖、乙醚提取物、没食子酸、原儿茶醛、儿茶素、表儿茶素、钙(Ca)、钠(Na)、镁(Mg)、硫(S)、铁(Fe)、锰(Mn)、硒(Se)和镍(Ni)的变异系数均超过36%,表明锁阳品质受生境影响显著。8种活性成分含量之间存在较强的协同作用和较弱的拮抗作用,12种矿质元素含量之间存在复杂的拮抗和协同作用。主成分分析表明,粗多糖、熊果酸、儿茶素、表儿茶素和总黄酮可作为评价锁阳品质的特征成分,钠、铜(Cu)、锰和镍是评价锁阳品质的特征元素。聚类分析中,以主要活性成分为聚类中心的第二组在活性物质含量方面品质较好,以矿质元素为聚类中心的第二组在矿质元素开发利用方面具有较高潜力。本研究可为不同生境锁阳的资源评价和优良品种选育提供依据,为锁阳的栽培和鉴定提供参考。