Chen P C, Gaetjens E, Broome J D
Department of Pathology, State University of New York, Downstate Medical Center, Brooklyn 11203.
Agents Actions. 1987 Oct;22(1-2):171-5. doi: 10.1007/BF01968834.
Buthionine sulfoximine depleted the glutathione (GSH) level of mouse lymphoma L1210A cells in culture to 6% of control and killed the cells within 48 hours in medium supplemented with fetal calf serum or bovine serum albumin. Mercaptoethanol or alpha-thioglycerol but not GSH or cysteine added to the medium protected the cells from the effect of GSH depletion. Horse serum was also protective, and this effect was removed by dialysis over 65 hours and could not be restored by adding GSH. Mercaptoethanol alone had a protective action in the dialyzed sera. The results suggest that mercaptoethanol may act independently and perform the functions of GSH.
丁硫氨酸亚砜亚胺可使培养的小鼠淋巴瘤L1210A细胞的谷胱甘肽（GSH）水平降至对照水平的6%，并在添加胎牛血清或牛血清白蛋白的培养基中于48小时内杀死细胞。向培养基中添加巯基乙醇或α-硫甘油而非GSH或半胱氨酸可保护细胞免受GSH耗竭的影响。马血清也具有保护作用，这种作用经65小时透析后消失，且添加GSH无法恢复。单独的巯基乙醇在透析血清中具有保护作用。结果表明，巯基乙醇可能独立发挥作用并执行GSH的功能。
