Moghaddam Akbar Hajizadeh, Parhizgar Maryam, Jelodar Sedigheh Khanjani, Eslami Ali, Sayrafi Reza
Department of Animal Science, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
Department of Veterinary Basic Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Psychopharmacology (Berl). 2023 May;240(5):1063-1074. doi: 10.1007/s00213-023-06344-4. Epub 2023 Mar 6.
Antioxidant natural herb hesperetin (Hst) offers powerful medicinal properties. Despite having noticeable antioxidant properties, it has limited absorption, which is a major pharmacological obstacle.
The goal of the current investigation was to determine if Hst and nano-Hst might protect mice against oxidative stress and schizophrenia (SCZ)-like behaviors brought on by ketamine (KET).
Seven treatment groups (n=7) were created for the animals. For 10 days, they received distilled water or KET (10 mg/kg) intraperitoneally (i.p). From the 11th to the 40th day, they received daily oral administration of Hst and nano-Hst (10, 20 mg/kg) or vehicle. With the use of the forced swimming test (FST), open field test (OFT), and novel object recognition test (NORT), SCZ-like behaviors were evaluated. Malondialdehyde (MDA) and glutathione levels and antioxidant enzyme activities were assessed in the cerebral cortex.
Our findings displayed that behavioral disorders induced by KET would be improved by nano-Hst treated. MDA levels were much lower after treatment with nano-Hst, and brain antioxidant levels and activities were noticeably higher. The mice treated with nano-Hst had improved outcomes in the behavioral and biochemical tests when compared to the Hst group.
Our study's findings showed that nano-Hst had a stronger neuroprotective impact than Hst. In cerebral cortex tissues, nano-Hst treatment dramatically reduced KET-induced (SCZ)-like behavior and oxidative stress indicators. As a result, nano-Hst may have more therapeutic potential and may be effective in treating behavioral impairments and oxidative damage brought on by KET.
抗氧化天然草药橙皮素（Hst）具有强大的药用特性。尽管具有显著的抗氧化特性，但其吸收有限，这是一个主要的药理学障碍。
本研究的目的是确定Hst和纳米Hst是否可以保护小鼠免受氯胺酮（KET）引起的氧化应激和精神分裂症（SCZ）样行为的影响。
为动物创建了七个治疗组（n = 7）。它们连续10天腹腔注射（i.p.）蒸馏水或KET（10 mg/kg）。从第11天到第40天，它们每天口服Hst和纳米Hst（10、20 mg/kg）或赋形剂。使用强迫游泳试验（FST）、旷场试验（OFT）和新物体识别试验（NORT）评估SCZ样行为。评估大脑皮层中的丙二醛（MDA）和谷胱甘肽水平以及抗氧化酶活性。
我们的研究结果表明，纳米Hst治疗可改善KET诱导的行为障碍。纳米Hst治疗后MDA水平显著降低，大脑抗氧化水平和活性显著升高。与Hst组相比，纳米Hst治疗的小鼠在行为和生化测试中表现更好。
我们的研究结果表明，纳米Hst比Hst具有更强的神经保护作用。在大脑皮层组织中，纳米Hst治疗显著降低了KET诱导的（SCZ）样行为和氧化应激指标。因此，纳米Hst可能具有更大的治疗潜力，可能对治疗KET引起的行为障碍和氧化损伤有效。
