Research Centre of Plant Protection, Yunnan Institute of Tropical Crops, Jinghong 666100, China.
Department of Chemistry and Biology, Xingyi Normal University for Nationalities, Xingyi 562400, China.
Plant Dis. 2023 Oct;107(10):3037-3050. doi: 10.1094/PDIS-11-22-2685-RE. Epub 2023 Oct 18.
Among the most damaging diseases of rubber trees is anthracnose caused by the genus , which leads to significant economic losses. Nonetheless, the specific spp. that infect rubber trees in Yunnan Province, an important natural rubber base in China, have not been extensively investigated. Here, we isolated 118 strains from rubber tree leaves exhibiting anthracnose symptoms in multiple plantations in Yunnan. Based on comparisons of their phenotypic characteristics and internal transcribed spacer ribosomal DNA sequences, 80 representative strains were chosen for additional phylogenetic analysis based on eight loci (, , cal, , , , , and ), and nine species were identified. , , and were found to be the dominant pathogens causing rubber tree anthracnose in Yunnan. was common, whereas , , , , and were rare. Among these nine species, and are reported for the first time in China, and two species are new to the world: sp. nov. in the species complex and sp. nov. in the species complex. Their pathogenicity was confirmed with Koch's postulates by inoculating each species in vivo on rubber tree leaves. This study clarifies the geographic distribution of spp. associated with anthracnose on rubber trees in representative locations of Yunnan, which is crucial for the implementation of quarantine measures.
在橡胶树最具破坏性的疾病中，炭疽病是由属引起的，这导致了重大的经济损失。然而，在中国重要的天然橡胶基地云南省感染橡胶树的具体 spp. 尚未得到广泛研究。在这里，我们从云南省多个种植园中表现出炭疽病症状的橡胶树叶片中分离出了 118 株菌株。基于表型特征和内部转录间隔区核糖体 DNA 序列的比较，选择了 80 株具有代表性的菌株，基于 8 个基因座（、、cal、、、、和）进行了额外的系统发育分析，鉴定了 9 个种。发现、和是引起云南橡胶树炭疽病的主要病原菌。是常见的，而、、、、和则很少见。在这 9 个种中，和在中国是首次报道的，而有两个种是世界上新的：种复合体中的 sp. nov. 和 种复合体中的 sp. nov.。通过在橡胶树叶片上体内接种每种物种，用柯赫氏假设证实了它们的致病性。本研究阐明了与云南代表性地区橡胶树炭疽病相关的 spp. 的地理分布，这对于实施检疫措施至关重要。
