State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, China.
Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
J Biomater Appl. 2023 Apr;37(9):1593-1604. doi: 10.1177/08853282231163758. Epub 2023 Mar 15.
Decellularized extracellular matrix is one form of natural material in tissue engineering. The process of dECM retains the tissue microstructure, provides good cell adhesion sites, maintains most of biological signals that promotes the survival and differentiation ability of cells. In this study, sheep kidney was decellularized followed by histochemical staining, elemental analysis and scanning electron microscopy characterizations. The dECM scaffold was prepared with different sequences of freeze drying technology, crosslinking and the water absorption, porosity, mechanical strength with subsequent thermogravimetric analysis, Infrared spectroscopy and biocompatibility tests. Our results indicated that these decellularized treatments of sheep kidney can effectively remove DNA and retain uniform pore size distribution. After crosslinking the scaffold's water absorption decreased from 987.56 ± 40.21% to 934.39 ± 39.61%, the porosity decreased from 89.64 ± 3.2% to 85.09 ± 17.63%, and the compression modulus increased from 304.32 ± 25.43 kPa to 459.53 ± 38.92 kPa, with thermal process the percentage of weight loss decreased from 66.57% to 44.731%, in addition, the composition didn't change significantly, crosslinking could also promote the stability. In terms of biocompatibility, the number of viable cells increased significantly with the days. In conclusion, the crosslinked decellularized sheep kidney extracellular matrix scaffold reduced water absorption and porosity slightly, but has a significant increase in mechanical properties, and presented excellent biocompatibility which are beneficial to cell adhesion, growth and differentiation.
去细胞细胞外基质是组织工程中天然材料的一种形式。dECM 的处理过程保留了组织的微观结构，提供了良好的细胞附着位点，并维持了大多数促进细胞存活和分化能力的生物信号。在这项研究中，对绵羊肾脏进行去细胞处理，然后进行组织化学染色、元素分析和扫描电子显微镜表征。使用不同序列的冻干技术、交联和水吸收制备 dECM 支架，随后进行热重分析、红外光谱和生物相容性测试，以评估其多孔性、机械强度和水吸收能力。我们的结果表明，这些绵羊肾脏的去细胞处理可以有效地去除 DNA，并保持均匀的孔径分布。交联后，支架的水吸收从 987.56 ± 40.21%降低到 934.39 ± 39.61%，孔隙率从 89.64 ± 3.2%降低到 85.09 ± 17.63%，压缩模量从 304.32 ± 25.43 kPa 增加到 459.53 ± 38.92 kPa，热过程中的重量损失百分比从 66.57%降低到 44.731%，此外，组成没有明显变化，交联还可以提高稳定性。就生物相容性而言，随着时间的推移，活细胞数量显著增加。总之，交联的去细胞绵羊肾细胞外基质支架略微降低了水吸收和孔隙率，但机械性能显著提高，并且表现出良好的生物相容性，有利于细胞黏附、生长和分化。
