To explore the best freeze-drying technology of Polygonatum sibiricum delar. Ex redoute and to establish the best HPLC method for the determination of main effective components of it from different areas in China.

To establish the freeze-drying process and the HPLC method to determine the main effective components and content of freeze-dried of China. The results show that the freeze-drying process is a slice thickness at 3 mm, boiling time 2 min, pre-freezing temperature at -35 °C, low-temperature holding at -10 °C, low temperature sublimation at 10 °C and drying temperature at 35 °C. The optimum HPLC conditions of freeze-dried was SepaxGp-C18 (4.6 × 250 mm, 5 μm), gradient elution of mobile phase A (acetonitrile)-B (ultra-pure water), detection wavelength of 254 nm, the flow rate of 1 mL/min, column temperature of at 30 °C and injection volume of 10 μL. from different producing areas contains a variety of amino acids, diosgenin, sugars, and other active ingredients. The protein content of from Yunnan Pu'er is the highest among them, and that of Shangluo in Shaanxi is the lowest. The total sugar content of in Puer is the highest, while that of in Wenshan is the lowest. And the diosgenin of in Lijiang is the highest. In this study, the freeze-drying process of was established, and the main effective components and contents of freeze-dried was determined as the better HPLC method, provided theoretical and technical support for the rational development and utilization of .