Centro Nacional de Recursos Genéticos-INIFAP, Blvd. de la Biodiversidad No. 400, C.P. 47600 Tepatitlán de Morelos, Jalisco, México.
Campo Experimental Centro Altos de Jalisco-CIRPAC-INIFAP, Blvd. de la Biodiversidad No. 2470, C.P. 47600 Tepatitlán de Morelos, Jalisco, México.
J Microbiol Methods. 2023 Apr;207:106708. doi: 10.1016/j.mimet.2023.106708. Epub 2023 Mar 20.
There is an increased interest for finding strains able to contribute to plant nutrition and health, since these are desirable for the formulation of agricultural bioinoculants. Obtaining a safe and efficient product requires exhaustive evaluations from which most methods used for this purpose involve the use of substrates or are established under uncontrolled conditions, so that various factors can mask the results of the plant-microorganism interaction. In vitro methods mostly involve the use of Petri Dishes (PD) but limit the results to seed germination. Other methods of germination involve the use of acrylic boxes (GB) allowing for better plant development, but are little known. Methods such as ISTA are widely used to evaluate the physiological quality of seeds in productive terms. Despite their efficiency, these methods have not been previously used to evaluate the effect of plant-microorganism interaction on crops. In the present study, modifications were made to the germination between paper of ISTA (BP) method, and were compared to the PD anf GB methods to evaluate the impact of the bacterium Serratia liquefaciens 385 and the yeast Clavispora lusitaniae Y35 on maize, bean and squash. Through the evaluation of physiological parameters in seed and seedling, the results clearly showed the superiority of the BP method to evaluate the effect of microorganisms since it allows observing a better development in the seedlings in terms of growth of the plumule, a better architecture of the radical system in which the emergence of adventitious secondary roots and differentiated radical hairs is observed in comparison with seedlings obtained under the other methods. Similarly, it was possible to observe the different effects on each of the three crops with respect to the inoculation of the bacteria and yeast. These results were significantly better in seedlings obtained in the BP method independently of the type of crop evaluated, considering the BP method suitable to be applied in large-scale bioprospecting plant-growth-promoting microorganism studies.
人们越来越感兴趣地寻找能够促进植物营养和健康的菌株，因为这些菌株是农业生物接种剂配方的理想选择。获得安全有效的产品需要进行详尽的评估，为此目的而使用的大多数方法都涉及使用基质或在不受控制的条件下进行，因此，各种因素可能会掩盖植物-微生物相互作用的结果。体外方法主要涉及使用培养皿 (PD)，但将结果限制在种子萌发上。其他萌发方法涉及使用丙烯酸盒 (GB)，这有利于更好的植物发育，但知之甚少。ISTA 等方法广泛用于从生产角度评估种子的生理质量。尽管这些方法效率很高，但以前没有用于评估植物-微生物相互作用对作物的影响。在本研究中，对 ISTA 纸间萌发 (BP) 方法进行了修改，并与 PD 和 GB 方法进行了比较，以评估细菌液化沙雷氏菌 385 和酵母鲁氏克鲁维酵母 Y35 对玉米、豆类和南瓜的影响。通过对种子和幼苗生理参数的评估，结果清楚地表明 BP 方法在评估微生物影响方面具有优越性，因为它可以观察到幼苗在胚芽生长方面更好的发育，根系系统的结构更好，在幼苗中观察到不定次生根和分化根毛的出现，而与其他方法获得的幼苗相比。同样，可以观察到接种细菌和酵母对三种作物的不同影响。在 BP 方法中，无论评估的作物类型如何，与其他方法相比，幼苗的生长情况都明显更好，这表明 BP 方法适合应用于大规模生物勘探植物生长促进微生物研究。
