探究两亲性丙烯酸盐共聚物与牛血清白蛋白的行为和动力学研究。
Probing the behavior and kinetic studies of amphiphilic acrylate copolymers with bovine serum albumin.
机构信息
Department of Chemistry, School of Natural Sciences (SNS), National University of Sciences and Technology, H-12, Islamabad, 44000, Pakistan.
Chemical Engineering & Material Science, Michigan State University, East Lansing, MI, 48824, USA.
出版信息
Sci Rep. 2023 Mar 20;13(1):4572. doi: 10.1038/s41598-023-27515-5.
This article presents that acrylate copolymers are the potential candidate against the adsorption of bovine serum albumin (BSA). A series of copolymers poly(methyl methacrylate) (pMMA), poly(3-sulfopropyl methacrylate-co-methyl methacrylate) p(SPMA-co-MMA), and poly(dimethylaminoethyl methacrylate-co-methyl methacrylate) p(DMAEMA-co-MMA) were synthesized via free radical polymerization. These amphiphilic copolymers are thermally stable with a glass transition temperature (Tg) 50-120 °C and observed the impact of surface charge on amphiphilic copolymers to control interactions with the bovine serum albumin (BSA). These copolymers pMD1 and pMS1 have surface charges, - 56.6 and - 72.6 mV at pH 7.4 in PBS buffer solution that controls the adsorption capacity of bovine serum albumin (BSA) on polymers surface. Atomic force microscopy (AFM) analysis showed minimum roughness of 0.324 nm and 0.474 nm for pMS1 and pMD1. Kinetic studies for BSA adsorption on these amphiphilic copolymers showed the best fitting of the pseudo-first-order model that showed physisorption and attained at 25 °C and pH 7.4 within 24 h.
本文提出，丙烯酸盐共聚物是对抗牛血清白蛋白（BSA）吸附的潜在候选物。通过自由基聚合合成了一系列共聚物：聚（甲基丙烯酸甲酯）（pMMA）、聚（3-磺丙基甲基丙烯酸酯-共-甲基丙烯酸甲酯）p（SPMA-co-MMA）和聚（二甲基氨基乙基甲基丙烯酸酯-共-甲基丙烯酸甲酯）p（DMAEMA-co-MMA）。这些两亲共聚物具有热稳定性，玻璃化转变温度（Tg）为 50-120°C，并观察到表面电荷对两亲共聚物的影响，以控制其与牛血清白蛋白（BSA）的相互作用。这些共聚物 pMD1 和 pMS1 在 pH 7.4 的 PBS 缓冲溶液中带有表面电荷，分别为-56.6 和-72.6 mV，可以控制牛血清白蛋白（BSA）在聚合物表面的吸附量。原子力显微镜（AFM）分析表明，pMS1 和 pMD1 的最小粗糙度分别为 0.324nm 和 0.474nm。BSA 在这些两亲共聚物上的吸附动力学研究表明，准一级模型的拟合效果最佳，表明在 25°C 和 pH 7.4 下，在 24 小时内达到了物理吸附。
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