The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Talanta. 2023 Jun 1;258:124466. doi: 10.1016/j.talanta.2023.124466. Epub 2023 Mar 18.
This paper proposed a hand-powered centrifugal micropipette-tip strategy, termed HCM, for all-in-one immunoassay combined with a distance-based readout for portable quantitative detection of SARS-CoV-2. The target SARS-CoV-2 virus antigen triggers the binding of multiple monoclonal antibody-coated red latex nanobeads, forming larger complexes. Following incubation and centrifugation, the formed aggregated complexes settle at the bottom of the tip, while free red nanobeads remain suspended in the solution. The HCM enables sensitive (1 ng/mL) and reliable quantification of SARS-CoV-2 within 25 min. With the advantages of free washing, free fabrication, free instrument, and without the optical device, the proposed low-cost and easy-to-use HCM immunoassay shows great potential for quantitative POC diagnostics for SARS-CoV-2.
本文提出了一种手动离心微量管尖端策略（HCM），用于一体式免疫分析，并结合基于距离的读出，用于便携式定量检测 SARS-CoV-2。目标 SARS-CoV-2 病毒抗原触发多个涂有单克隆抗体的红色乳胶纳米珠的结合，形成更大的复合物。孵育和离心后，形成的聚集复合物沉淀在尖端底部，而游离的红色纳米珠仍悬浮在溶液中。HCM 能够在 25 分钟内实现 SARS-CoV-2 的灵敏（1ng/mL）和可靠定量。由于具有免清洗、免制造、免仪器以及无光学器件的优势，所提出的低成本、易于使用的 HCM 免疫分析方法对于 SARS-CoV-2 的即时诊断具有很大的潜力。
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