Guo Meng-Yao, Li Kai-Yu, Nie Min-Hai, Liu Xu-Qian
Department of Periodontal Mucosal Diseases, the Affiliated Stomatological Hospital of Southwest Medical University. Luzhou 646000, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2023 Feb;32(1):40-46.
To explore the effect of overexpression of DCN（decorin） gene on the expression of epidermal growth factor receptor (EGFR), cellular-myelocytomatosis viral oncogene (C-Myc) and cyclin dependent kinase inhibitor (p21)in tumor-bearing nude mice with oral squamous cell carcinoma（OSCC）.
The expression of DCN gene in human oral squamous cell carcinoma(HSC-3) was up-regulated by liposome transfection. Nude mice were used as the carrier of OSCC. H-E staining was used to determine the pathological grade of tumor-bearing tissues in each group. Immunohistochemistry was used to detect the expression of EGFR, C-Myc and p21 protein in tumor-bearing tissues of each group after DCN overexpression. RT-qPCR and Western blot were used to quantitatively detect the expression of EGFR, C-Myc and p21 in tumor-bearing tissues of each group after DCN overexpression, and to determine the effects of DCN overexpression on the expression of EGFR, C-Myc and p21 in tumor-bearing tissues of OSCC nude mice. SPSS 20.0 software package was used for statistical analysis.
H-E staining showed that the animal model of OSCC was successfully constructed. The tumor-bearing tissues of nude mice in the plasmid group were significantly lighter than those in the empty vector group and non-transfected group(P＜0.05). IHC results showed that DCN, EGFR, C-Myc and p21 proteins were expressed in the tumor-bearing tissues of nude mice in each group, the expression of DCN,EGFR and C-Myc proteins in the plasmid group was significantly different from the other groups(P＜0.05).There was no significant differece in p21 protein expression in each group(P＞0.05). RT-qPCR and Western blot results showed that DCN, EGFR, C-Myc and p21 were expressed in diffrent degrees in tumor-bearing tissues of nude mice（P＜0.05）.
DCN can inhibit the growth of tumor in OSCC nude mice. In tumor-bearing tissues of nude mice with OSCC, overexpression of DCN can down-regulate the expression of EGFR and C-Myc, and up-regulate the expression of p21.DCN may play an inhibitory role in the occurrence and development of OSCC.
探讨核心蛋白聚糖（DCN）基因过表达对荷口腔鳞状细胞癌（OSCC）裸鼠表皮生长因子受体（EGFR）、细胞髓细胞瘤病毒癌基因（C-Myc）和细胞周期蛋白依赖性激酶抑制剂（p21）表达的影响。
采用脂质体转染上调人口腔鳞状细胞癌（HSC-3）中DCN基因的表达。以裸鼠作为OSCC的载体。采用苏木精-伊红（H-E）染色确定各组荷瘤组织的病理分级。采用免疫组织化学法检测DCN过表达后各组荷瘤组织中EGFR、C-Myc和p21蛋白的表达。采用逆转录-定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹法（Western blot）定量检测DCN过表达后各组荷瘤组织中EGFR、C-Myc和p21的表达，以确定DCN过表达对OSCC裸鼠荷瘤组织中EGFR、C-Myc和p21表达的影响。使用SPSS 20.0软件包进行统计分析。
H-E染色显示成功构建了OSCC动物模型。质粒组裸鼠的荷瘤组织明显比空载体组和未转染组轻（P＜0.05）。免疫组织化学结果显示，各组裸鼠荷瘤组织中均表达DCN、EGFR、C-Myc和p21蛋白，质粒组中DCN、EGFR和C-Myc蛋白的表达与其他组有显著差异（P＜0.05）。各组p21蛋白表达无显著差异（P＞0.05）。RT-qPCR和Western blot结果显示，DCN、EGFR、C-Myc和p21在裸鼠荷瘤组织中均有不同程度的表达（P＜0.05）。
DCN可抑制OSCC裸鼠肿瘤的生长。在荷OSCC裸鼠的荷瘤组织中，DCN过表达可下调EGFR和C-Myc的表达，并上调p21的表达。DCN可能在OSCC的发生发展中起抑制作用。
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