Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Independent Researcher, Tehran, Iran.
BMC Med Genomics. 2023 Mar 28;16(1):62. doi: 10.1186/s12920-023-01492-0.
Adult T-cell Leukemia/Lymphoma (ATLL) is a rapidly progressing type of T-cell non-Hodgkin lymphoma that is developed after the infection by human T-cell leukemia virus type 1 (HTLV-1). It could be categorized into four major subtypes, acute, lymphoma, chronic, and smoldering. These different subtypes have some shared clinical manifestations, and there are no trustworthy biomarkers for diagnosis of them.
We applied weighted-gene co-expression network analysis to find the potential gene and miRNA biomarkers for various ATLL subtypes. Afterward, we found reliable miRNA-gene interactions by identifying the experimentally validated-target genes of miRNAs.
The outcomes disclosed the interactions of miR-29b-2-5p and miR-342-3p with LSAMP in ATLL_acute, miR-575 with UBN2, miR-342-3p with ZNF280B, and miR-342-5p with FOXRED2 in ATLL_chronic, miR-940 and miR-423-3p with C6orf141, miR-940 and miR-1225-3p with CDCP1, and miR-324-3p with COL14A1 in ATLL_smoldering. These miRNA-gene interactions determine the molecular factors involved in the pathogenesis of each ATLL subtype and the unique ones could be considered biomarkers.
The above-mentioned miRNAs-genes interactions are suggested as diagnostic biomarkers for different ATLL subtypes.
成人 T 细胞白血病/淋巴瘤（ATLL）是一种由人类 T 细胞白血病病毒 1（HTLV-1）感染后发展而来的快速进展型 T 细胞非霍奇金淋巴瘤。它可以分为四大主要亚型，即急性、淋巴瘤、慢性和亚临床。这些不同的亚型有一些共同的临床表现，目前还没有可靠的生物标志物用于诊断。
我们应用加权基因共表达网络分析来寻找各种 ATLL 亚型的潜在基因和 miRNA 生物标志物。随后，我们通过鉴定 miRNA 的实验验证靶基因来确定可靠的 miRNA-基因相互作用。
研究结果揭示了 miR-29b-2-5p 和 miR-342-3p 与 LSAMP 在 ATLL_acute 中的相互作用，miR-575 与 UBN2 的相互作用，miR-342-3p 与 ZNF280B 的相互作用，miR-342-5p 与 FOXRED2 在 ATLL_chronic 中的相互作用，miR-940 和 miR-423-3p 与 C6orf141 的相互作用，miR-940 和 miR-1225-3p 与 CDCP1 的相互作用，以及 miR-324-3p 与 COL14A1 在 ATLL_smoldering 中的相互作用。这些 miRNA-基因相互作用决定了每种 ATLL 亚型发病机制中涉及的分子因素，而独特的相互作用可以被认为是生物标志物。
上述 miRNA-基因相互作用被认为是不同 ATLL 亚型的诊断生物标志物。
