Zhang Yuan, He Kai, Guo Xuhao, Jiang Jia, Qian Le, Xu Jianqiang, Che Zhiping, Huang Xiaobo, Liu Shengming
Department of Plant Protection, College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China.
National Key Laboratory of Veterinary Public Health Security and School of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
J Fungi (Basel). 2023 Mar 8;9(3):334. doi: 10.3390/jof9030334.
has been identified as a significant pathogen. It causes Fusarium crown rot (FCR), which occurs in several major wheat-producing areas in China. Chemical control is the primary measure with which to control this disease. In this study, transcriptome sequencing (RNA-Seq) was used to determine the different mechanisms of action of four frequently used fungicides including carbendazim, pyraclostrobin, tebuconazole, and phenamacril on . In brief, 381, 1896, 842, and 814 differentially expressed genes (DEGs) were identified under the carbendazim, pyraclostrobin, tebuconazole, and phenamacril treatments, respectively. After the joint analysis, 67 common DEGs were obtained, and further functional analysis showed that the ABC transported pathway was significantly enriched. Moreover, ( and (), two important ABC multidrug transporter genes whose expression levels simultaneously increased, were mined under the different treatments, which unambiguously demonstrated the common effects. In addition, Mfuzz clustering analysis and WGCNA analysis revealed that the core DEGs are involved in several critical pathways in each of the four treatment groups. Taken together, these genes may play a crucial function in the mechanisms of 's response to the fungicides stress.
已被确定为一种重要的病原体。它会引发镰刀菌冠腐病（FCR），该病在中国几个主要小麦产区均有发生。化学防治是控制这种病害的主要措施。在本研究中，利用转录组测序（RNA-Seq）来确定包括多菌灵、吡唑醚菌酯、戊唑醇和苯醚甲环唑在内的四种常用杀菌剂对……的不同作用机制。简而言之，在多菌灵、吡唑醚菌酯、戊唑醇和苯醚甲环唑处理下，分别鉴定出381、1896、842和814个差异表达基因（DEG）。联合分析后，获得了67个共同的DEG，进一步的功能分析表明ABC转运途径显著富集。此外，在不同处理下挖掘出了（和（），这两个重要的ABC多药转运蛋白基因，其表达水平同时升高，明确证明了共同效应。此外，Mfuzz聚类分析和WGCNA分析表明，核心DEG参与了四个处理组中每组的几个关键途径。综上所述，这些基因可能在……对杀菌剂胁迫的反应机制中发挥关键作用。
