Liu Lin, Jin Xiaofan, Lu Xiuhua, Guo Lizhong, Lu Peiwei, Yu Hao, Lv Beibei
College of Life Science, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China.
Bayer Crop Science China Co., Ltd., Hangzhou 310018, China.
J Fungi (Basel). 2023 Mar 17;9(3):367. doi: 10.3390/jof9030367.
wilt is a severe and worldwide disease in potato cultivation. In this study, was first identified as the pathogen of potato wilt. SF1 has the potential for controlling potato wilt induced by resulting in a mycelium growth inhibition of 52.50 ± 2.59% in vitro and a significant decrease in incidence rate by 45.56% in vivo. This research highlighted the antifungal activity of surfactin from SF1 and attempted to reveal the unknown antifungal mechanisms. Surfactin inhibited mycelium growth beyond the concentration of 20 μg/μL. Surfactin-treated mycelium appeared to have morphological malformation. Surfactin enhanced reduced glutathione production and caused the increase in values of the extracellular fluids in OD260 and OD280. Surfactin induced differential protein expression and changed the genes' transcription levels. Surfactin binds to fungal DNA via groove-binding mode, with a binding constant of 2.97 × 10 M. Moreover, SF1 harbored genes encoding plant-promoting determinants, making potato seedlings grow vigorously. The results will help provide a comprehensive understanding of the mechanisms of surfactin against filamentous fungi and the application of surfactin-producing microbial in the biocontrol of plant pathogenic fungi.
枯萎病是马铃薯种植中一种严重的全球性病害。在本研究中，首次鉴定出[具体名称未给出]为马铃薯枯萎病的病原菌。SF1具有控制由[病原菌名称未给出]引起的马铃薯枯萎病的潜力，在体外导致菌丝体生长抑制率为52.50±2.59%，在体内发病率显著降低45.56%。本研究突出了来自SF1的表面活性素的抗真菌活性，并试图揭示未知的抗真菌机制。表面活性素在浓度超过20μg/μL时抑制[病原菌名称未给出]菌丝体生长。经表面活性素处理的菌丝体似乎出现形态畸形。表面活性素增强了还原型谷胱甘肽的产生，并导致细胞外液在OD260和OD280处的值增加。表面活性素诱导差异蛋白表达并改变基因转录水平。表面活性素通过沟槽结合模式与真菌DNA结合，结合常数为2.97×10 M。此外，SF1含有编码促进植物生长决定因素的基因，使马铃薯幼苗生长旺盛。这些结果将有助于全面了解表面活性素对丝状真菌的作用机制以及产表面活性素微生物在植物病原真菌生物防治中的应用。
