犬蛔虫感染对比格犬肝脏长链非编码 RNA（lncRNA）和信使 RNA（mRNA）表达的调控。

Modulation of long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression in the liver of Beagle dogs by Toxocara canis infection.

机构信息

State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, People's Republic of China.

Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.

出版信息

Parasit Vectors. 2023 Mar 29;16(1):114. doi: 10.1186/s13071-023-05738-9.

DOI:10.1186/s13071-023-05738-9

PMID:36991462

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10057693/

Abstract

BACKGROUND

Long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) play crucial roles in regulating various physiological and pathological processes. However, the role of lncRNAs and mRNAs in mediating the liver response during Toxocara canis infection remains incompletely understood.

METHODS

In the present study, the expression profile of lncRNAs and mRNAs was investigated in the liver of Beagle dogs infected by T. canis using high-throughput RNA sequencing.

RESULTS

Compared with the control groups, 876 differentially expressed (DE) lncRNAs and 288 DEmRNAs were identified at 12 h post-infection (hpi), 906 DElncRNAs and 261 DEmRNAs were identified at 24 hpi, and 876 DElncRNAs and 302 DEmRNAs were identified at 36 days post-infection (dpi). A total of 16 DEmRNAs (e.g. dpp4, crp and gnas) were commonly identified at the three infection stages. Enrichment and co-localization analyses identified several pathways involved in immune and inflammatory responses during T. canis infection. Some novel DElncRNAs, such as LNC_015756, LNC_011050 and LNC_011052, were also associated with immune and inflammatory responses. Also, LNC_005105 and LNC_005401 were associated with the secretion of anti-inflammatory cytokines, which may play a role in the healing of liver pathology at the late stage of infection.

CONCLUSIONS

Our data provided new insight into the regulatory roles of lncRNAs and mRNAs in the pathogenesis of T. canis and improved our understanding of the contribution of lncRNAs and mRNAs to the immune and inflammatory response of the liver during T. canis infection.

摘要

背景

长链非编码 RNA（lncRNA）和信使 RNA（mRNA）在调控各种生理和病理过程中发挥着关键作用。然而，lncRNA 和 mRNA 在介导犬弓首蛔虫感染时肝脏反应中的作用仍不完全清楚。

方法

本研究采用高通量 RNA 测序技术，研究了犬弓首蛔虫感染后 Beagle 犬肝脏中 lncRNA 和 mRNA 的表达谱。

结果

与对照组相比，感染后 12 hpi 时鉴定出 876 个差异表达（DE）lncRNA 和 288 个 DEmRNA，24 hpi 时鉴定出 906 个 DElncRNA 和 261 个 DEmRNA，36 dpi 时鉴定出 876 个 DElncRNA 和 302 个 DEmRNA。在三个感染阶段共有 16 个 DEmRNA（如 dpp4、crp 和 gnas）被共同鉴定出来。富集和共定位分析鉴定了几个在犬弓首蛔虫感染过程中参与免疫和炎症反应的途径。一些新的 DElncRNA，如 LNC_015756、LNC_011050 和 LNC_011052，也与免疫和炎症反应有关。此外，LNC_005105 和 LNC_005401 与抗炎细胞因子的分泌有关，这可能在感染后期肝脏病理的愈合中发挥作用。