Rogier E, Cassio D, Weiss M C, Feldmann G
Differentiation. 1986;30(3):229-36. doi: 10.1111/j.1432-0436.1986.tb00785.x.
We compared the ultrastructure of a well-differentiated rat hepatoma line (H4II) and its clonal progeny, including dedifferentiated variant cells, and revertants of the variants in which the spectrum of hepatocyte-specific functions is again expressed. The cells of the original differentiated lines and the revertants were very similar to one another. In addition, they exhibited some of the characteristics of fetal and neonatal hepatocytes. Variant cells which fail to express hepatocyte functions showed a wide range of morphological alterations accompanied by generalized disorganization. It is concluded that the loss of hepatocyte differentiation in the variants is not associated with a uniform morphological type, and that a wide range of ultrastructural phenotypes can be generated in the progeny of a single neoplastic but well-differentiated hepatocyte. Also, the expression of hepatocyte functions only occurs within a limited and organized morphological framework that includes features of young hepatocytes.
我们比较了一种高分化大鼠肝癌细胞系（H4II）及其克隆后代的超微结构，其中包括去分化的变异细胞以及变异细胞的回复突变体，在这些回复突变体中再次表达了肝细胞特异性功能谱。原始分化细胞系和回复突变体的细胞彼此非常相似。此外，它们表现出一些胎儿和新生儿肝细胞的特征。未能表达肝细胞功能的变异细胞表现出广泛的形态学改变，并伴有普遍的结构紊乱。结论是，变异细胞中肝细胞分化的丧失与单一的形态学类型无关，并且在单个肿瘤性但高分化的肝细胞后代中可以产生广泛的超微结构表型。此外，肝细胞功能的表达仅发生在一个有限且有组织的形态学框架内，该框架包括年轻肝细胞的特征。
