Neurology Department, Hunan Academy of Chinese Medicine, Changsha 410006, China.
School of Humanities and Management, Hunan University of Chinese Medicine, Changsha 410208, China.
J Tradit Chin Med. 2023 Apr;43(2):295-302. doi: 10.19852/j.cnki.jtcm.20221108.002.
To evaluate the efficacy of Baishao Luoshi decoction (, BD) on synaptic plasticity in rats with post stroke spasticity (PSS), and to study the mechanism behind the action.
The PSS model of rat was established by middle cerebral artery occlusion (MCAO). The neurological deficit symptoms were evaluated by modified neurological deficit score (mNSS). Muscle tension were evaluated by Modified Ashworth score (MAS). Transmission electron microscopy (TEM) was used to observe the synaptic ultrastructure. The expression of synaptic plasticity-related protein brain derived neurotrophic factor (BDNF), growth associated protein-43 (GAP43), synaptophysin (p38) and microtubule-associated protein 2 (MAP2) in the brain tissue around the infarct were detected by Western blotting.
We found that mNSS were significantly improved and limb spasticity was ameliorated treated by BD. The thickness of postsynaptic density and the synaptic curvature increased significantly. The expression of synaptic plasticity-related protein BDNF, GAP43, p38, MAP2 in the brain tissue around the infarct were raised remarkably after treated by BD.
Alleviating PSS by BD may be related to rescuing the synaptic plasticity, which provides a probable new therapeutic method for PSS.
评价白芍蒺藜汤（BD）对脑卒中后痉挛（PSS）大鼠突触可塑性的疗效，并探讨其作用机制。
采用大脑中动脉闭塞（MCAO）法建立 PSS 大鼠模型。采用改良神经功能缺损评分（mNSS）评估神经功能缺损症状，采用改良 Ashworth 评分（MAS）评估肌肉张力。采用透射电镜（TEM）观察突触超微结构。采用 Western blot 法检测脑组织中与突触可塑性相关的蛋白脑源性神经营养因子（BDNF）、生长相关蛋白-43（GAP43）、突触小体相关蛋白（p38）和微管相关蛋白 2（MAP2）的表达。
BD 可显著改善 mNSS 评分，减轻肢体痉挛。突触后密度厚度和突触曲率明显增加。BD 处理后，梗死周围脑组织中与突触可塑性相关的蛋白 BDNF、GAP43、p38、MAP2 的表达明显升高。
BD 缓解 PSS 可能与挽救突触可塑性有关，为 PSS 提供了一种可能的新治疗方法。
