Goto Katsumasa, Ohashi Kazuya
Department of Physiology, Graduate School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan.
Methods Mol Biol. 2023;2640:217-225. doi: 10.1007/978-1-0716-3036-5_16.
The nerve transection model is an established and validated experimental model of skeletal muscle atrophy prepared by denervating the skeletal muscle in rodents. While a number of denervation techniques are available in rats, the development of various transgenic and knockout mice has also led to the wide use of mouse models of nerve transection. Skeletal muscle denervation experiments expand our knowledge of the physiological role of nerval activity and/or neurotrophic factors in the plasticity of skeletal muscle. The denervation of the sciatic or tibial nerve is a common experimental procedure in mice and rats, as these nerves can be resected without great difficulty. An increasing number of reports have recently been published on experiments using a tibial nerve transection technique in mice. In this chapter, we demonstrate and explain the procedures used to transect the sciatic and tibial nerves in mice.
神经切断模型是一种通过使啮齿动物的骨骼肌去神经支配而建立并经过验证的骨骼肌萎缩实验模型。虽然大鼠有多种去神经支配技术，但各种转基因和基因敲除小鼠的发展也导致神经切断小鼠模型得到广泛应用。骨骼肌去神经支配实验扩展了我们对神经活动和/或神经营养因子在骨骼肌可塑性中的生理作用的认识。坐骨神经或胫神经的去神经支配是小鼠和大鼠常见的实验操作，因为这些神经可以相对容易地进行切除。最近有越来越多关于在小鼠中使用胫神经切断技术进行实验的报道发表。在本章中，我们将演示并解释在小鼠中切断坐骨神经和胫神经所使用的操作步骤。
