miR-320b 异常表达通过上调 ALDH1A3 的表达增强人脑胶质瘤的放射抵抗性。
MiR-320b aberrant expression enhances the radioresistance of human glioma via upregulated expression of ALDH1A3.
机构信息
Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Department of Radiotherapy, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
出版信息
Aging (Albany NY). 2023 Mar 29;15(6):2347-2357. doi: 10.18632/aging.204617.
Accumulating evidence has demonstrated that ALDH1A3 is closely associated with development, progression, radioresistance and prognosis in a variety of cancers. However, the upstream miRNA that plays in the ALDH1A3 signaling pathways in regulating the radioresistance of glioma remains unclear. In this study, ALDH1A3 was enriched in high-grade glioma and was determined to be essential for radioresistance in GBM cell lines. Moreover, miR-320b was identified as an upstream miRNA that interacts with ALDH1A3. Low expression of miR-320b was associated with poor prognosis and radioresistance in glioma. In addition, overexpression of miR-320b counteracted the effects of ALDH1A3 on GBM cell proliferation, apoptosis and radioresistance when exposed to X-ray irradiation. Collectively, miR-320b may serve as a novel therapeutic target for glioma patients.
越来越多的证据表明，ALDH1A3 与多种癌症的发生、发展、放射抵抗和预后密切相关。然而，在调节胶质瘤放射抵抗的 ALDH1A3 信号通路中起作用的上游 miRNA 尚不清楚。在这项研究中，ALDH1A3 在高级别胶质瘤中富集，并被确定为 GBM 细胞系放射抵抗所必需。此外，miR-320b 被鉴定为与 ALDH1A3 相互作用的上游 miRNA。miR-320b 的低表达与胶质瘤患者的不良预后和放射抵抗相关。此外，当暴露于 X 射线照射时，miR-320b 的过表达可逆转 ALDH1A3 对 GBM 细胞增殖、凋亡和放射抵抗的影响。综上所述，miR-320b 可能成为治疗胶质瘤患者的新靶点。
Zotero 插件