Dubey J P, Miller S, Powell E C, Anderson W R
J Am Vet Med Assoc. 1986 Jan 15;188(2):155-8.
During the lambing season of 1983/1984, 8 of 44 purebred Hampshire ewes on a farm in Knoxville, Md had reproductive problems. In at least 4 of these ewes, the problem was attributed to toxoplasmosis. Necrosis and Toxoplasma gondii tachyzoites were found in placental specimens from 3 ewes. Agglutinating antibody to T gondii, at a titer of 1:80, was found in pleural fluids of both fetuses aborted from 1 ewe; this ewe had an antibody titer of 1:6,400 at the time of abortion. In another ewe, the diagnosis was confirmed by the isolation of T gondii from the placenta and 1 of her lambs. Of numerous free-roaming adult cats on the farm, 16 were trapped, euthanatized, and examined for T gondii. Agglutination antibody to T gondii, at titers of 1:4 to 1:64, was found in serum samples from all the cats. Toxoplasma gondii was isolated from the brain and skeletal muscles of 9 of the cats, and from the feces of 1 cat. Blood samples obtained from all 78 sheep on the farm 6 months after the episode of abortion were examined for antibody to T gondii. Agglutinating antibody titers to T gondii were less than 1:16 in 46 sheep, 1:16 in 16, 1:64 in 12, 1:256 in 2, 1:024 in 1, and 1:4,096 in 1. Analyses of serologic data in sheep of various age groups suggested that the Toxoplasma infection was acquired sporadically, probably from feed contaminated with oocysts.
在1983/1984年的产羔季节，马里兰州诺克斯维尔一个农场的44只纯种汉普郡母羊中有8只出现了生殖问题。在这些母羊中，至少有4只的问题被归因于弓形虫病。在3只母羊的胎盘标本中发现了坏死和刚地弓形虫速殖子。从1只母羊流产的2个胎儿的胸水中发现了效价为1:80的抗弓形虫凝集抗体；这只母羊流产时抗体效价为1:6400。在另一只母羊中，从胎盘及其1只羔羊中分离出弓形虫，从而确诊。该农场有许多自由放养的成年猫，捕获了16只，实施安乐死后检查是否感染弓形虫。在所有猫的血清样本中均发现了效价为1:4至1:64的抗弓形虫凝集抗体。从9只猫的脑和骨骼肌以及1只猫的粪便中分离出了弓形虫。在流产事件发生6个月后，对农场所有78只绵羊采集血样检测抗弓形虫抗体。46只绵羊的抗弓形虫凝集抗体效价低于1:16，16只绵羊为1:16，12只为1:64，2只为1:256，1只为1:1024，1只为1:4096。对不同年龄组绵羊血清学数据的分析表明，弓形虫感染是零星发生的，可能是通过被卵囊污染的饲料感染的。
