Division of Laboratory Science, Niigata University Graduate School of Health Sciences, Niigata, Japan.
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Nat Commun. 2023 Mar 31;14(1):1817. doi: 10.1038/s41467-023-37399-8.
Human parechovirus (PeV-A) is an RNA virus that belongs to the family Picornaviridae and it is currently classified into 19 genotypes. PeV-As usually cause mild illness in children and adults. Among the genotypes, PeV-A3 can cause severe diseases in neonates and young infants, resulting in neurological sequelae and death. In this study, we identify the human myeloid-associated differentiation marker (MYADM) as an essential host factor for the entry of six PeV-As (PeV-A1 to PeV-A6), including PeV-A3. The infection of six PeV-As (PeV-A1 to PeV-A6) to human cells is abolished by knocking out the expression of MYADM. Hamster BHK-21 cells are resistant to PeV-A infection, but the expression of human MYADM in BHK-21 confers PeV-A infection and viral production. Furthermore, VP0 capsid protein of PeV-A3 interacts with one extracellular domain of human MYADM on the cell membrane of BHK-21. The identification of MYADM as an essential entry factor for PeV-As infection is expected to advance our understanding of the pathogenesis of PeV-As.
人类副肠孤病毒（PeV-A）是一种 RNA 病毒，属于小 RNA 病毒科，目前分为 19 种基因型。PeV-A 通常在儿童和成人中引起轻微疾病。在这些基因型中，PeV-A3 可导致新生儿和婴幼儿发生严重疾病，导致神经后遗症和死亡。在本研究中，我们鉴定出人类髓系相关分化标记物（MYADM）是六种 PeV-A（PeV-A1 至 PeV-A6，包括 PeV-A3）进入宿主细胞的必需宿主因子。敲除 MYADM 的表达可消除六种 PeV-A（PeV-A1 至 PeV-A6）对人细胞的感染。仓鼠 BHK-21 细胞对 PeV-A 感染具有抗性，但在 BHK-21 中表达人 MYADM 可赋予 PeV-A 感染和病毒产生能力。此外，PeV-A3 的 VP0 衣壳蛋白与人 BHK-21 细胞膜上的 MYADM 的一个细胞外结构域相互作用。鉴定出 MYADM 是 PeV-A 感染的必需进入因子，有望增进我们对 PeV-A 发病机制的理解。
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