Central Veterinary Research Laboratory, Dubai, United Arab Emirates.
Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
Parasitol Res. 2023 Jun;122(6):1317-1325. doi: 10.1007/s00436-023-07831-5. Epub 2023 Apr 1.
The lifecycle of Brachymeria podagrica, a parasitic wasp with a worldwide distribution, was studied under laboratory conditions using the flesh fly, Sarcophaga dux, as a host. Two hundred parasite-free 3rd instars of S. dux were exposed for 24 h to 20 female B. podagrica. In daily intervals, maggots and later pupae were examined for developmental stages of the parasitoid. The whole pre-imaginal development at a temperature of 26 °C lasted 21 to 26 days. Three morphologically different instars, followed by a prepupal and a pupal stage, were described using light and scanning electron microscopy. In a second experiment with 100 3rd stage Sarcophaga larvae and 10 parasitoids, a total of 70 wasps emerged 20 to 25 days after exposure. Two fly larvae did not pupate and dried out, while 28 pupae contained a dry or caseous content, dead wasp imagos, or their larval stages. No fly imagines emerged from exposed groups, while all 100 unexposed larvae pupated and adults eclosed between day 12 and day 14 after the start of the experiment, while the imagoes of the parasitoids appeared 8 to 12 days later.
研究了分布于全球的寄生蜂 Brachymeria podagrica 的生命周期,实验条件下以肉蝇 Sarcophaga dux 为宿主。将 200 只无寄生虫的 3 龄 S. dux 幼虫暴露于 20 只雌性 B. podagrica 中 24 小时。每隔一天,检查幼虫和后来的蛹,以观察寄生蜂的发育阶段。在 26°C 的温度下,整个预蛹期持续 21 至 26 天。使用光学显微镜和扫描电子显微镜描述了 3 个形态不同的龄期,接着是预蛹期和蛹期。在第二个实验中,将 100 只 3 龄 Sarcophaga 幼虫和 10 只寄生蜂暴露于实验中,暴露后 20 至 25 天共出现了 70 只黄蜂。2 只蝇幼虫未化蛹而干枯,而 28 只蛹中含有干的或干酪状物质、死亡的黄蜂成虫或其幼虫阶段。暴露组中没有出现蝇成虫,而所有 100 只未暴露的幼虫在实验开始后第 12 至 14 天化蛹并成虫出壳,而寄生蜂的成虫则在 8 至 12 天后出现。