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基于赫拉克斯尼奥超快速气相电子鼻的不同来源郁金饮片快速识别及鉴别标志物研究

[Rapid identification and differential markers of Curcumae Radix decoction pieces of different sources based on Heracles Neo ultra-fast gas phase electronic nose].

作者信息

Li Ming-Xuan, Qin Yu-Wen, Li Yu, Zhang Jiu-Ba, Ji De, Qu Ling-Yun, Gong Jing-Wen, Jia Ao-Meng, Mao Chun-Qin, Lu Tu-Lin

机构信息

School of Pharmacy,Nanjing University of Chinese Medicine Nanjing 210023,China.

School of Pharmacy,Nanjing University of Chinese Medicine Nanjing 210023,China Jiangsu Provincial Engineering Research Center of TCM External Medication Development and Application Nanjing 210023,China Traditional Chinese Medicine Processing Technology Inheritance Base of the State Administration of Traditional Chinese Medicine Nanjing 210023,China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2023 Mar;48(6):1518-1525. doi: 10.19540/j.cnki.cjcmm.20221203.301.

Abstract

Since Curcumae Radix decoction pieces have multiple sources, it is difficult to distinguish depending on traditional cha-racters, and the mixed use of multi-source Curcumae Radix will affect its clinical efficacy. Heracles Neo ultra-fast gas phase electronic nose was used in this study to quickly identify and analyze the odor components of 40 batches of Curcumae Radix samples from Sichuan, Zhejiang, and Guangxi. Based on the odor fingerprints established for Curcumae Radix decoction pieces of multiple sources, the odor components was identified and analyzed, and the chromatographic peaks were processed and analyzed to establish a rapid identification method. Principal component analysis(PCA), discriminant factor analysis(DFA), and soft independent modeling cluster analysis(SIMCA) were constructed for verification. At the same time, one-way analysis of variance(ANOVA) combined with variable importance in projection(VIP) was employed to screen out the odor components with P<0.05 and VIP>1, and 13 odor components such as β-caryophyllene and limonene were hypothesized as the odor differential markers of Curcumae Radix decoction pieces of diffe-rent sources. The results showed that Heracles Neo ultra-fast gas phase electronic nose can well analyze the odor characteristics and rapidly and accurately discriminate Curcumae Radix decoction pieces of different sources. It can be applied to the quality control(e.g., online detection) in the production of Curcumae Radix decoction pieces. This study provides a new method and idea for the rapid identification and quality control of Curcumae Radix decoction pieces.

摘要

由于郁金饮片来源多样,依靠传统性状难以鉴别,多来源郁金混用会影响其临床疗效。本研究采用Heracles Neo超快速气相电子鼻对来自四川、浙江、广西的40批次郁金样品的气味成分进行快速识别与分析。基于多来源郁金饮片建立的气味指纹图谱,对气味成分进行识别与分析,并对色谱峰进行处理与分析,建立快速鉴别方法。构建主成分分析(PCA)、判别因子分析(DFA)和软独立建模聚类分析(SIMCA)进行验证。同时,采用单因素方差分析(ANOVA)结合投影变量重要性(VIP)筛选出P<0.05且VIP>1的气味成分,推测β-石竹烯、柠檬烯等13种气味成分作为不同来源郁金饮片的气味差异标志物。结果表明,Heracles Neo超快速气相电子鼻能够很好地分析气味特征,快速准确地鉴别不同来源的郁金饮片,可应用于郁金饮片生产中的质量控制(如在线检测)。本研究为郁金饮片的快速鉴别和质量控制提供了新的方法和思路。

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