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牦牛肉对烫伤大鼠日粮促进伤口愈合的作用。

Effect of Yak Meat to the Daily Ration of Scalded Rats for Wound Healing.

作者信息

Wang Hong-Jin, Feng Yan-Ping, Tian Xiao-Xia, Wu Xiao-Han, Hao Li-Zhuang, Li Yi, Mei Shi-Juan

机构信息

Department of Burns and Plastic Surgery, Affiliated Hospital of Qinghai University, Xining, Qinghai, 810012, People's Republic of China.

Qinghai University, Academy of Animal Science and Veterinary Medicine, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Xining, Qinghai, 810016, People's Republic of China.

出版信息

Clin Cosmet Investig Dermatol. 2023 Mar 29;16:751-767. doi: 10.2147/CCID.S393066. eCollection 2023.

Abstract

OBJECTIVE

Treatment of burn wound healing involves infection, nutrition, psychology and rehabilitation, and proper nutritional support can promote wound healing, enhance immune function and reduce the incidence of complications. This study aimed to investigate the effects of feed containing yak meat on scalded rats' body condition and wound healing.

METHODS

Adopting a two-factor factorial design, the growth performance, food intake, body weight, and Lee's index of rats were measured. The wound conditions of scalded rats with different feeds (basic, basic + yak meat, and basic + yellow beef) were observed at different periods, and their wounds' hematoxylin and eosin (H&E) staining states were detected. The proliferating cell nuclear antigen (PCNA)-positive cells and apoptosis were analyzed to evaluate the effects of feed on the wound healing of scalded rats.

RESULTS

The feed intake was the highest in the yellow beef feed group and the lowest in the yak meat feed group. The body weight was the highest in the yak meat feed group and the lowest in the yellow beef feed group. Furthermore, 45 days after scalding, the obesity index in the yak beef feed group was the closest to that of the rats before scalding. The wound recovery of the rats in the yak meat feed group was the best at 30 days, and the H&E staining results also proved that the recovery effect of the scalded rats in the yak meat feed group was better than other two groups. According to the results of PCNA and apoptosis, the yak meat feed group had lower positive cell rate and faster wound healing.

CONCLUSION

The rats in the yak meat feed group recovered better than those in the other groups, and the yak beef feed had the best effect on the wound healing of the scalded rats.

摘要

目的

烧伤创面愈合的治疗涉及感染、营养、心理及康复等方面,合理的营养支持可促进创面愈合、增强免疫功能并降低并发症的发生率。本研究旨在探讨含牦牛肉饲料对烫伤大鼠身体状况及创面愈合的影响。

方法

采用两因素析因设计,测定大鼠的生长性能、采食量、体重及李氏指数。观察不同饲料(基础饲料、基础+牦牛肉饲料、基础+黄牛肉饲料)喂养的烫伤大鼠在不同时期的创面情况,并检测其创面苏木精-伊红(H&E)染色状态。分析增殖细胞核抗原(PCNA)阳性细胞及凋亡情况,以评估饲料对烫伤大鼠创面愈合的影响。

结果

黄牛肉饲料组大鼠采食量最高,牦牛肉饲料组最低。牦牛肉饲料组大鼠体重最高,黄牛肉饲料组最低。此外,烫伤45天后,牦牛肉饲料组大鼠的肥胖指数最接近烫伤前。牦牛肉饲料组大鼠在30天时创面恢复情况最佳,H&E染色结果也证明牦牛肉饲料组烫伤大鼠的恢复效果优于其他两组。根据PCNA及凋亡结果,牦牛肉饲料组阳性细胞率较低,创面愈合较快。

结论

牦牛肉饲料组大鼠恢复情况优于其他组,牦牛肉饲料对烫伤大鼠创面愈合效果最佳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f217/10066901/abf786575f2e/CCID-16-751-g0001.jpg

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