Kokal W, Sheibani K, Terz J, Harada J R
JAMA. 1986 Jun 13;255(22):3123-7.
Tumor DNA content has been described as having prognostic significance in patients with colorectal carcinoma. It has been unclear, however, whether tumor ploidy as a prognostic factor is independent of various standard prognostic variables such as depth of invasion and lymph node involvement by the tumor. We retrospectively examined 77 patients who were diagnosed between 1974 and 1980 as having had resectable Dukes' stage A, B, or C colorectal carcinomas. The DNA content of each tumor was analyzed by flow cytometry on paraffin-embedded specimens. Both for aneuploid stage B tumors and for the entire group of aneuploid tumors, the disease-free and overall survival times of the patients were significantly shorter than those of patients with diploid colorectal carcinomas. Logistic regression analysis demonstrated that aneuploidy was an independent prognostic variable in predicting recurrent disease as well as death from the colorectal cancer. Tumor ploidy was, in fact, the single most important prognostic factor among all of the clinical and pathologic variables studied. Thus, the DNA content of colorectal carcinoma appears to play an important role in indicating the biologic aggressiveness of the disease.
肿瘤DNA含量已被描述为对结直肠癌患者具有预后意义。然而，肿瘤倍体作为一种预后因素是否独立于各种标准预后变量，如肿瘤浸润深度和淋巴结受累情况，一直尚不清楚。我们回顾性研究了1974年至1980年间被诊断为可切除的杜克A、B或C期结直肠癌的77例患者。通过对石蜡包埋标本进行流式细胞术分析每个肿瘤的DNA含量。无论是非整倍体B期肿瘤还是整个非整倍体肿瘤组，患者的无病生存期和总生存期均明显短于二倍体结直肠癌患者。逻辑回归分析表明，非整倍体是预测复发性疾病以及结直肠癌死亡的独立预后变量。事实上，在所有研究的临床和病理变量中，肿瘤倍体是最重要的单一预后因素。因此，结直肠癌的DNA含量似乎在表明该疾病的生物学侵袭性方面发挥着重要作用。
