Wilkening R B
Proc Soc Exp Biol Med. 1986 Jun;182(2):237-44. doi: 10.3181/00379727-182-42334.
The purpose of this study was to determine whether samples from the veins of the pregnant and the nonpregnant horn of the uterus lead to similar estimates of uterine blood flow and oxygen consumption. To accomplish this, a comparison of uterine blood flow, arteriovenous differences of oxygen content, and oxygen consumption measured by sampling the venous drainages of the two uterine horns was performed on eight pregnant sheep during the last 20 days of pregnancy. Each sheep carried a single fetus. Umbilical and uterine blood flows were measured with the test substances ethanol and antipyrine by application of the steady-state diffusion method. Twenty-three measurements of uterine blood flow comparing the two horns were not significantly different (P greater than 0.1), and were highly correlated (r = 0.98). The ratio of the oxygen content arteriovenous difference in the pregnant to that in the nonpregnant horn and the ratio of the uterine blood flow in the nonpregnant to that in the pregnant horn were significantly correlated (r = 0.7). As a consequence, paired calculations of oxygen consumption for the whole pregnant uterus had a small coefficient of variation (+/- 3.7%). These results demonstrate that the use of highly diffusible test substances for the measurement of uterine blood flow in pregnant sheep can provide accurate data for the calculation of uterine oxygen uptake, in part because the oxygen and test substance molecules are similarly affected by local variations in placental perfusion.
本研究的目的是确定从怀孕子宫角和未怀孕子宫角的静脉采集的样本，是否能得出相似的子宫血流和氧消耗估计值。为实现这一目的，在怀孕最后20天对8只怀孕绵羊进行了研究，通过对两个子宫角的静脉引流进行采样，比较子宫血流、氧含量的动静脉差值以及氧消耗。每只绵羊怀有一个胎儿。采用稳态扩散法，用测试物质乙醇和安替比林测量脐血流和子宫血流。比较两个子宫角的23次子宫血流测量结果无显著差异（P大于0.1），且相关性很高（r = 0.98）。怀孕子宫角与未怀孕子宫角的氧含量动静脉差值之比，以及未怀孕子宫角与怀孕子宫角的子宫血流之比显著相关（r = 0.7）。因此，对整个怀孕子宫的氧消耗进行配对计算时，变异系数较小（±3.7%）。这些结果表明，使用高扩散性测试物质测量怀孕绵羊的子宫血流，可为计算子宫氧摄取提供准确数据，部分原因是氧分子和测试物质分子受胎盘灌注局部变化的影响相似。
