山杏总黄酮对大鼠佐剂性关节炎的作用及自噬的调节。
Effects of Prunus Tomentosa Thumb Total Flavones on adjuvant arthritis in rats and regulation of autophagy.
机构信息
College of Basic Medicine, Beihua University, Jilin, China.
State Key Laboratory of Quality Research in Chinese Medicine, Macao University of Science and Technology, Macao, China.
出版信息
Technol Health Care. 2023;31(S1):123-136. doi: 10.3233/THC-236012.
BACKGROUND
Rheumatoid arthritis (RA) is a slow in taking effect systemic autoimmune disease. Prunus Tomentosa Thumb Total Flavones (PTTTF) has anti-inflammatory and antioxidant properties.
OBJECTIVE
The purpose of this study is to the PTTTF on adjuvant arthritis (AA) in rats and to explore the mechanism of autophagy.
METHODS
Adjuvant arthritis model was established in rats. The cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-17 (IL-17), tumor necrosis factor (TNF-α) of rat synovial tissue were determined by RT-PCR. The histopathological varieties of knee joints in AA rats were observed by HE staining. The expressions of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2 in rat synovial tissue were determined by Western Blotting.
RESULTS
PTTTF (50, 100, 200 mg/kg) significantly inhibited inflammation in rats (P< 0.01). PTTTF significantly inhibited inflammatory factor COX in rat synovial tissue. COX-2, IL-1β, IL-6, IL-17, TNF-α expression (P< 0.05); PTTTF can significantly improve the pathological damage of rat knee joint PTTTF and can significantly inhibited the expression of autophagy-related proteins in rat synovium (P< 0.05 ).
CONCLUSION
PTTTF can inhibit adjuvant arthritis in rats and can inhibit the expression of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2.
背景
类风湿关节炎（RA）是一种起效缓慢的系统性自身免疫性疾病。山杏总黄酮（PTTTF）具有抗炎和抗氧化作用。
目的
本研究旨在观察山杏总黄酮（PTTTF）对佐剂性关节炎（AA）大鼠的作用，并探讨其自噬机制。
方法
建立大鼠佐剂性关节炎模型。采用 RT-PCR 法检测大鼠滑膜组织中环氧化酶 1（COX-1）和环氧化酶 2（COX-2）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-17（IL-17）、肿瘤坏死因子（TNF-α）的表达。HE 染色观察 AA 大鼠膝关节组织的病理变化。Western blot 法检测大鼠滑膜组织中自噬相关蛋白 ATG5、ATG7、ATG12、Beclin1、Lc3II 和 Bcl-2 的表达。
结果
PTTTF（50、100、200mg/kg）能显著抑制大鼠炎症（P<0.01）。PTTTF 能显著抑制大鼠滑膜组织中炎性因子 COX 的表达。COX-2、IL-1β、IL-6、IL-17、TNF-α的表达（P<0.05）；PTTTF 能明显改善大鼠膝关节的病理损伤，PTTTF 能明显抑制大鼠滑膜组织中自噬相关蛋白的表达（P<0.05）。
结论
PTTTF 能抑制佐剂性关节炎大鼠，抑制自噬相关蛋白 ATG5、ATG7、ATG12、Beclin1、Lc3II 和 Bcl-2 的表达。
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