Department of Ophthalmology, Nippon Medical School, Tokyo 113-8602, Japan.
Department of Analytic Human Pathology, Nippon Medical School, Tokyo 113-8602, Japan.
Int J Mol Sci. 2023 Mar 26;24(7):6254. doi: 10.3390/ijms24076254.
Zinn's zonule is a fragile and thin tissue, and little is known about its pathogenesis. The aim of this study was to develop an experimental setup for a comprehensive analysis of Zinn's zonule. Rats were divided into two groups: a control group ( = 4) and an alkali injury group ( = 4). Seven days after injury, the eyes were enucleated, the anterior eye was dissected and embedded in gelatin, and macroscopic observations were made. The gelatin specimens were then embedded in paraffin and observed in detail by low-vacuum scanning electron microscopy, immunofluorescence, and quantitative reverse transcription polymerase chain reaction (RT-qPCR). The results show qualitative changes in Zinn's zonules in both macroscopic and microscopic observations. In addition, macrophage infiltration and increased matrix metalloproteinase 2 (MMP2) expression were observed in the injured group, consistent with the RT-qPCR results. The experimental system in this study allowed us to capture the morphological and molecular biological changes of Zinn's zonule and to gain insight into its pathogenesis. In conclusion, this study presents a new experimental setup for the comprehensive analysis of the rat Zinn's zonule. The results suggest that this system can be used in the future to study and analyze a variety of paraffin-embedded tissues and specimens.
津恩氏带是一种脆弱而薄的组织，其发病机制知之甚少。本研究旨在建立一种用于全面分析津恩氏带的实验装置。将大鼠分为两组：对照组（n=4）和碱损伤组（n=4）。损伤后 7 天，眼球被摘除，前眼被解剖并嵌入明胶中，进行宏观观察。然后将明胶标本嵌入石蜡中，通过低真空扫描电子显微镜、免疫荧光和定量逆转录聚合酶链反应（RT-qPCR）进行详细观察。结果表明，在宏观和微观观察中，津恩氏带均发生了定性变化。此外，在损伤组中观察到巨噬细胞浸润和基质金属蛋白酶 2（MMP2）表达增加，与 RT-qPCR 结果一致。本研究中的实验系统允许我们捕捉津恩氏带的形态和分子生物学变化，并深入了解其发病机制。总之，本研究提出了一种用于全面分析大鼠津恩氏带的新实验装置。研究结果表明，该系统未来可用于研究和分析各种石蜡包埋组织和标本。
