Cao Jiaxing, Xu Ning, Zhang Jianhao, Zhang Guozhi, Zhang Yu
College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Foods. 2023 Mar 24;12(7):1376. doi: 10.3390/foods12071376.
It is essential to understand the mechanism of action of ultrasound synergistic free radical oxidation to promote covalent reactions between proteins and polyphenols. (-)-epigallo-catechin 3-gallate (EGCG) with rich bioactivity could be used to increase the functional properties of cereal protein-gliadin (GL). This study systematically explored the role of ultrasound treatment (US) on the binding mechanisms of GL and EGCG. Electrophoresis and high-performance liquid chromatography (HPLC) confirmed the greater molecular mass of the covalent complexes in the ultrasound environment. Quantitative analysis by the phenol content revealed that the ultrasound environment increased the EGCG content in the covalent complex by 15.08 mg/g of protein. The changes in the spatial structure of the proteins were indicated by Fourier infrared and ultraviolet spectroscopy. Additionally, scanning electron microscopy (SEM) and atomic force microscopy (AFM) found that US disrupted the aggregation of GL and the clustered structure of the covalent complexes. The results demonstrated that the water solubility of ultrasonic conjugates was significantly increased by 8.8-64.19%, the digestion rate was more efficient, and the radical scavenging capacity was twice that of GL. This research contributes to the theoretical basis for broadening the application of polyphenols in modifying protein.
了解超声协同自由基氧化促进蛋白质与多酚之间共价反应的作用机制至关重要。具有丰富生物活性的（-）-表没食子儿茶素-3-没食子酸酯（EGCG）可用于提高谷物蛋白-麦醇溶蛋白（GL）的功能特性。本研究系统地探讨了超声处理（US）对GL与EGCG结合机制的作用。电泳和高效液相色谱（HPLC）证实了超声环境中共价复合物的分子量更大。通过酚含量进行的定量分析表明，超声环境使共价复合物中的EGCG含量增加了15.08 mg/g蛋白质。傅里叶红外光谱和紫外光谱表明了蛋白质空间结构的变化。此外，扫描电子显微镜（SEM）和原子力显微镜（AFM）发现，超声处理破坏了GL的聚集以及共价复合物的聚集结构。结果表明，超声偶联物的水溶性显著提高了8.8-64.19%，消化率更高，自由基清除能力是GL的两倍。本研究为拓宽多酚在蛋白质改性中的应用提供了理论依据。
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