基于豌豆分离蛋白/果胶的白藜芦醇包封复合物的制备、表征及乳化性能
Fabrication, characterization, and emulsifying properties of complex based on pea protein isolate/pectin for the encapsulation of pterostilbene.
作者信息
Tang Zonghui, Tao Yuting, Huang Qiuye, Huang Yousheng, Huang Jun, Wu Yisu, Jing Xinyu, Yang Tao, Li Xueling, Liang Jin, Sun Yue
机构信息
Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Anhui Engineering Laboratory for Agro-products Processing, School of Tea & Food Science, Anhui Agricultural University, Hefei, China.
Anhui Province Key Laboratory of Analysis and Detection for Food Safety, Technical Center for Hefei Customs, Hefei, China.
出版信息
Food Chem X. 2023 Mar 27;18:100663. doi: 10.1016/j.fochx.2023.100663. eCollection 2023 Jun 30.
In this study, pectin (PEC) and pea protein isolate(PPI) was successfully used to create complexes as a novel delivery system for pterostilbene (PT). When the mass ratio of PEC to PPI was 0.5, the particle size and ζ-potential of PPI-PEC-PT were 119.41 ± 5.68 nm and -23.26 ± 0.61 mV, respectively, and the encapsulation efficiency (EE) of PT was 90.92 ± 2.08%. The photochemical stability of PT was enhanced after encapsulation. The results of the molecular docking and multispectral analysis demonstrated that the PPI and PT binding was spontaneous and mostly fueled by hydrophobic interactions. The hydrophobicity of PPI was significantly decreased and the emulsification activity and emulsion stability were significantly improved after production with PEC and PT. The best emulsification impact was demonstrated by the PPI-PEC-PT complex. PPI-PEC is an effective PT delivery material, and the PPI-PEC-PT complex is a new functional emulsification material with significant potential in liquid and semi-liquid food and health products.
在本研究中，果胶（PEC）和豌豆分离蛋白（PPI）成功用于制备复合物，作为芪三酚（PT）的新型递送系统。当PEC与PPI的质量比为0.5时，PPI-PEC-PT的粒径和ζ电位分别为119.41±5.68nm和-23.26±0.61mV，PT的包封率（EE）为90.92±2.08%。包封后PT的光化学稳定性增强。分子对接和多光谱分析结果表明，PPI与PT的结合是自发的，主要由疏水相互作用驱动。与PEC和PT结合后，PPI的疏水性显著降低，乳化活性和乳液稳定性显著提高。PPI-PEC-PT复合物表现出最佳的乳化效果。PPI-PEC是一种有效的PT递送材料，PPI-PEC-PT复合物是一种新型功能性乳化材料，在液体和半液体食品及保健品中具有巨大潜力。
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