Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AW Groningen, the Netherlands.
Nanoscale. 2023 May 4;15(17):7920-7928. doi: 10.1039/d3nr01167d.
Lipid peroxidation is a process that occurs in cells when they are exposed to oxidative stress. During the process reactive oxygen species attack lipids within the lipid bilayers of cells. Since the products of lipid peroxidation are toxic and carcinogenic, it is important to understand where and how it occurs with nanoscale resolution. The radical intermediates of this process are particularly interesting since they are causing chain reactions damaging large parts of the lipid membranes in cells. However, they are also difficult to measure for the state of the art because they are short lived and reactive. Here, we study the lipid peroxidation of three artificial lipid bilayers on a diamonds substrate that can be used to study lipid peroxidation. In particular, we present a diamond quantum sensing method called -relaxometry that allows for measurements and imaging of radical intermediates of lipid peroxidation in these membranes.
脂质过氧化是细胞在暴露于氧化应激时发生的一种过程。在这个过程中，活性氧物质攻击细胞脂质双分子层中的脂质。由于脂质过氧化的产物是有毒和致癌的，因此了解它在纳米尺度下发生的位置和方式非常重要。这个过程的自由基中间体特别有趣，因为它们引发链式反应，破坏细胞中大部分的脂质膜。然而，由于它们的寿命短且具有反应性，因此对于现有技术来说，它们也很难被测量。在这里，我们研究了三种人工脂质双层在金刚石衬底上的脂质过氧化，这些人工脂质双层可用于研究脂质过氧化。特别是，我们提出了一种称为弛豫率测量的金刚石量子传感方法，该方法允许在这些膜中测量和成像脂质过氧化的自由基中间体。
