Bulger R E, Burke T J, Cronin R E, Schrier R W, Dobyan D C
Anat Rec. 1986 Apr;214(4):341-7. doi: 10.1002/ar.1092140402.
The 40-minute infusion of norepinephrine (NE) into the renal artery of dogs produces a reversible ischemic model of acute renal failure. While the physiology of this model has been extensively studied, no complete description of the pathology exists. This study uses light microscopy and transmission electron microscopy to describe and quantitate the structural and ultrastructural changes which occur in the kidneys of dogs 1, 3, and 24 hours after the intrarenal infusion of 0.75 mg/kg/minute of NE. One hour after a 40-minute NE infusion the majority of convoluted and straight proximal tubules showed apical blebs, loss of brush border, microvillar whorl formation, and mitochondrial condensation and high-amplitude swelling with flocculent densities. Necrotic cells were occasionally seen at 1 hour. The injury was progressive after 3 hours and by 24 hours animals had either complete or partial patchy necrosis of all regions of the proximal tubule. The percentages of injured and necrotic proximal tubules in outer, mid-, and inner cortical regions are presented. We conclude that the extent and pattern of injury seen after NE infusion differs significantly from the renal artery clamping model of ischemia.
向犬肾动脉内输注去甲肾上腺素（NE）40分钟可产生急性肾衰竭的可逆性缺血模型。虽然对该模型的生理学进行了广泛研究，但尚无关于病理学的完整描述。本研究使用光学显微镜和透射电子显微镜来描述和定量在肾内输注0.75mg/kg/分钟NE后1小时、3小时和24小时犬肾脏中发生的结构和超微结构变化。在40分钟的NE输注后1小时，大多数曲部和直部近端小管出现顶端小泡、刷状缘丧失、微绒毛漩涡形成以及线粒体凝聚和高幅度肿胀并伴有絮状密度。1小时时偶尔可见坏死细胞。3小时后损伤呈进行性发展，到24小时时，动物近端小管所有区域出现完全或部分片状坏死。给出了皮质外层、中层和内层区域中受损和坏死近端小管的百分比。我们得出结论，NE输注后所见损伤的程度和模式与肾动脉夹闭缺血模型有显著差异。
