梅森-辉瑞猴病毒和松鼠猴D型逆转录病毒中脂质与蛋白质的化学交联

Chemical crosslinking of lipids and proteins within Mason-Pfizer monkey and squirrel monkey type D retroviruses.

作者信息

Uckert W, Rudolph M

出版信息

Arch Geschwulstforsch. 1986;56(2):107-12.

Abstract

Nearest neighborhoods between lipids and proteins were investigated in Mason-Pfizer monkey virus (MPMV) and squirrel monkey retrovirus (SMRV) using the chemical crosslinking reagent dimethyl suberimidate (DMS). Analysis of DMS-induced lipid-protein complexes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that in both viruses the major internal structural protein, p27 (MPMV) and p35 (SMRV), is linked to the lipid bilayer. Additionally, in SMRV a second protein (p20) was found to have a vicinal relationship to the lipid bilayer of the virus. Electron microscopic investigations of MPMV and SMRV particles indicated that, in accord with the crosslinking data, the core shell of these type D retroviruses is in close proximity to the viral membrane.

摘要

使用化学交联试剂辛二酸二甲酯亚胺（DMS）研究了梅森 - 辉瑞猴病毒（MPMV）和松鼠猴逆转录病毒（SMRV）中脂质与蛋白质之间最近的邻域关系。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳对DMS诱导的脂质 - 蛋白质复合物进行分析，结果显示在这两种病毒中，主要的内部结构蛋白，即MPMV的p27和SMRV的p35，都与脂质双层相连。此外，在SMRV中发现第二种蛋白质（p20）与病毒的脂质双层存在邻近关系。对MPMV和SMRV颗粒的电子显微镜研究表明，与交联数据一致，这些D型逆转录病毒的核心壳层与病毒膜紧密相邻。