Borensztajn J, Kotlar T J, Matza C A
Biochem J. 1986 Feb 1;233(3):909-12. doi: 10.1042/bj2330909.
Apoprotein-free heparin-binding and non-binding chylomicrons were used as substrates to test the effects on lipoprotein lipase activity of (a) chylomicron protein I; (b) the mixture of proteins I, II and apoprotein E and (c) human beta 2-glycoprotein I. No activation of the enzyme was observed with any of those apoproteins. When rats were injected simultaneously with [3H]cholesterol-labelled heparin-binding chylomicrons (containing proteins I and II) and [14C]cholesterol-labelled non-binding chylomicrons, no differences were detected between the rates of removal from circulation of those two types of particles. Clearance of chylomicrons from circulation was accompanied by the incorporation of 3H and 14C labels into the livers at similar rates. It is concluded that proteins I, II and apoprotein E have no effect on the degradation of chylomicrons by lipoprotein lipase and that the hepatic recognition of remnants does not appear to be affected by proteins I and II.
无载脂蛋白的肝素结合型和非结合型乳糜微粒被用作底物，以测试以下物质对脂蛋白脂肪酶活性的影响：(a) 乳糜微粒蛋白I；(b) 蛋白I、II和载脂蛋白E的混合物；(c) 人β2-糖蛋白I。使用这些载脂蛋白均未观察到该酶的激活。当给大鼠同时注射[3H]胆固醇标记的肝素结合型乳糜微粒（含蛋白I和II）和[14C]胆固醇标记的非结合型乳糜微粒时，未检测到这两种类型颗粒从循环中清除速率的差异。乳糜微粒从循环中的清除伴随着3H和14C标记以相似速率掺入肝脏。得出的结论是，蛋白I、II和载脂蛋白E对脂蛋白脂肪酶降解乳糜微粒没有影响，并且残余物的肝脏识别似乎不受蛋白I和II的影响。
