测量体外分化的小鼠原代棕色脂肪细胞中的线粒体解偶联

Measuring Mitochondrial Uncoupling in Mouse Primary Brown Adipocytes Differentiated Ex Vivo.

作者信息

Veliova Michaela, Tol Marcus J, Tontonoz Peter, Shirihai Orian S, Liesa Marc

机构信息

Division of Endocrinology, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

出版信息

Methods Mol Biol. 2023;2662:53-65. doi: 10.1007/978-1-0716-3167-6_5.

DOI:10.1007/978-1-0716-3167-6_5

PMID:37076670

Abstract

Measuring the mitochondrial respiratory capacity of brown adipocytes ex vivo is an essential approach to understand the cell-autonomous regulators of mitochondrial uncoupling in brown adipose tissue. Here, we describe two protocols to isolate brown preadipocytes from mice, their ex vivo differentiation to mature brown adipocytes and the quantification of their mitochondrial uncoupling capacity by respirometry.

摘要

体外测量棕色脂肪细胞的线粒体呼吸能力是了解棕色脂肪组织中线粒体解偶联的细胞自主调节因子的重要方法。在此，我们描述了两种从小鼠中分离棕色前脂肪细胞、将其体外分化为成熟棕色脂肪细胞以及通过呼吸测定法对其线粒体解偶联能力进行定量的方案。