鉴定和表征……中的基因。（原文不完整，“in”后面缺少具体内容）

Identification and characterization of genes in .

作者信息

Liu Weili, Ren Weichao, Liu Xiubo, He Lianqing, Qin Chen, Wang Panpan, Kong Lingyang, Li Yang, Liu Yunwei, Ma Wei

机构信息

School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China.

Experimental Teaching and Training Center, Heilongjiang University of Chinese Medicine, Harbin, China.

出版信息

Front Plant Sci. 2023 Apr 3;14:1152685. doi: 10.3389/fpls.2023.1152685. eCollection 2023.

DOI:10.3389/fpls.2023.1152685

PMID:37077646

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10106723/

Abstract

INTRODUCTION

genes encode plant-specific transcription factors, which regulate various biological processes such as growth, development, and secondary metabolite accumulation.

METHODS

We conducted whole-genome analysis of Chinese dwarf cherry () to identify genes and characterize the structure, motif composition, cis-acting elements, chromosomal distribution, and collinearity of these genes as well as the physical and chemical properties, amino acid sequences, and phylogenetic evolution of the encoded proteins.

RESULTS

The results revealed the presence of 25 genes in genome. All 25 genes could be divided into eight groups, and the members of the same group had similar motif arrangement and intron-exon structure. Promoter analysis showed that cis-acting elements responsive to abscisic acid, low temperature stress, and light were dominant. Transcriptome data revealed that most genes exhibited tissue-specific expression. Then, we performed by qRT-PCR to analyze the expression patterns of all 25 genes in fruit during storage. The results indicated that these genes exhibited different expression patterns, suggesting that they played an important role in fruit storage.

DISCUSSION

The results of this study provide a basis for further investigation of the biological function of genes in fruit.

摘要

引言

基因编码植物特异性转录因子，其调控各种生物学过程，如生长、发育和次生代谢产物积累。

方法

我们对郁李进行了全基因组分析，以鉴定基因，表征这些基因的结构、基序组成、顺式作用元件、染色体分布和共线性，以及编码蛋白质的物理化学性质、氨基酸序列和系统发育进化。

结果

结果显示郁李基因组中存在25个基因。所有25个基因可分为八组，同一组的成员具有相似的基序排列和内含子-外显子结构。启动子分析表明，对脱落酸、低温胁迫和光响应的顺式作用元件占主导。转录组数据显示，大多数基因呈现组织特异性表达。然后，我们通过qRT-PCR分析了所有25个基因在果实贮藏期间的表达模式。结果表明这些基因呈现不同的表达模式，表明它们在果实贮藏中发挥重要作用。