Bhardwaj Nandini, Nangia Rajat, Puri Abhiney, Bhat Nitish, Wadhwan Vijay, Gupta Hitesh
Department of Oral Pathology, Microbiology and Forensic Odontology, Himachal Institute of Dental Sciences, Paonta Sahib, Himachal Pradesh, India.
Conservative Dentistry and Endodontics, Himachal Institute of Dental Sciences, Paonta Sahib, Himachal Pradesh, India.
J Oral Maxillofac Pathol. 2022 Oct-Dec;26(4):488-494. doi: 10.4103/jomfp.jomfp_250_22. Epub 2022 Dec 22.
The determination of gender by dental pulp Barr bodies seems to be efficient tool especially in case of natural calamities where bodies are mutilated, charred, and unidentified. Different techniques are used for Barr bodies two of which are histopathological and cytological methods.
The objective of the study is to check the reliability of human dental pulp for identification of gender using Barr bodies.
The study sample consisted of 60 extracted teeth from 20 male and 40 female patients aged 12-25 years. Inclusion criteria included non-carious freshly extracted teeth, Teeth were extracted and the pulp was obtained using a carborundum disc at 30,000 revolutions per minute (RPM). The teeth were categorized into two groups. Group 1 and Group II include 20 females and 10 males each. In group I, rapid manual tissue processing technique was done and in Group II pulp tissue slides were prepared by cytopathological technique. The slides obtained were stained with H&E and Barr bodies were visualized.
On comparison of Barr bodies on males and females in dental pulp, the mean number of Barr body count in females was more than males. Accuracy and count were much more appreciated in histopathological technique than cytological procedure.
The mean Barr body count was more in females than in male samples. In histopathological technique Barr bodies were more analyzed and appreciated than in cytopathological technique on checking the efficacy and evaluating its diagnostic significance of dental pulp for identification of gender.
通过牙髓巴氏小体来确定性别似乎是一种有效的方法，尤其是在自然灾害导致尸体残缺、烧焦且身份不明的情况下。用于检测巴氏小体的技术有多种，其中两种是组织病理学方法和细胞学方法。
本研究的目的是检验利用巴氏小体通过人牙髓鉴定性别的可靠性。
研究样本包括从20名男性和40名年龄在12至25岁的女性患者身上提取的60颗牙齿。纳入标准包括非龋坏的新鲜拔除牙齿。牙齿被拔除后，使用碳化硅盘以每分钟30000转（RPM）的速度获取牙髓。牙齿被分为两组。第一组和第二组每组分别包括20名女性和10名男性。在第一组中，采用快速手动组织处理技术，在第二组中，通过细胞病理学技术制备牙髓组织切片。获得的切片用苏木精-伊红染色，观察巴氏小体。
比较牙髓中男性和女性的巴氏小体，女性的巴氏小体平均数量多于男性。在组织病理学技术中，准确性和计数比细胞学方法更受认可。
女性样本中的巴氏小体平均数量多于男性样本。在检查牙髓用于性别鉴定的有效性并评估其诊断意义时，与细胞病理学技术相比，组织病理学技术对巴氏小体的分析和观察更清晰。
