将AF64A-苦味磺酸盐注射到大鼠背侧海马体后，大鼠前脑胆碱能神经元变性的免疫组织化学证据。
Immunohistochemical evidence for degeneration of cholinergic neurons in the forebrain of the rat following injection of AF64A-picrylsulfonate into the dorsal hippocampus.
作者信息
Tonnaer J A, Lammers A J, Wieringa J H, Steinbusch H W
出版信息
Brain Res. 1986 Apr 2;370(1):200-3. doi: 10.1016/0006-8993(86)91125-x.
The cholinergic neurotoxin, AF64A-picrylsulfonate, was unilaterally infused into the dorsal hippocampus of Wistar rats (2 nmol/2 microliters/4 min; A 6.2, Ls 1.5, H 6.5, Paxinos and Watson). After 19 days the for immunohistochemical staining of choline acetyltransferase (ChAT). Morphometry and counting of ChAT-immunoreactive profiles revealed shrinkage and disappearance of cholinergic neurons in the medial septum and diagonal band of Broca at the lesioned brain side. These data indicate a retrograde degeneration of cholinergic neurons following injection of AF64A-picrylsulfonate into the dorsal hippocampus of the rat.
将胆碱能神经毒素AF64A - 苦味磺酸盐单侧注入Wistar大鼠的背侧海马体（2纳摩尔/2微升/4分钟；A 6.2，Ls 1.5，H 6.5，帕西诺斯和沃森图谱）。19天后进行胆碱乙酰转移酶（ChAT）的免疫组织化学染色。对ChAT免疫反应性轮廓进行形态计量和计数发现，损伤脑侧的内侧隔核和布罗卡斜带中的胆碱能神经元出现萎缩和消失。这些数据表明，将AF64A - 苦味磺酸盐注入大鼠背侧海马体后，胆碱能神经元发生逆行性变性。
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