School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, China.
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, China; Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo, Province, 315211, China.
Dev Comp Immunol. 2023 Aug;145:104714. doi: 10.1016/j.dci.2023.104714. Epub 2023 Apr 19.
The interleukin-6 family of cytokines possesses a diversity of roles with significant redundancy. The roles of these molecules have been relatively well characterized in mammals, with limited attention in other species. Progress has been made in the discovery of homologous molecules in fish. Here we report the characterization of pro-inflammatory properties of recombinant goldfish M17. Recombinant goldfish M17 enhanced phagocytosis, primed production of reactive oxygen intermediates, and was chemotactic to macrophages. Treatment of goldfish macrophages with LPS, heat-killed and live Aeromonas hydrophila resulted in higher M17 mRNA levels. Recombinant M17 (RgM17) induced dose-dependent production of IFNγ and IL-1β1 in goldfish macrophages. Furthermore, treatment of macrophages with rgM17 resulted in upregulation of transcription factors that were important in the differentiation of myeloid progenitors into monocytes/macrophages (Runx1 and GATA2). Our results indicate that goldfish M17 is an essential inflammatory cytokine for proliferation and differentiation of goldfish progenitor cells.
白细胞介素-6 家族细胞因子具有多种作用，具有显著的冗余性。这些分子的作用在哺乳动物中已经得到了相对较好的描述，而在其他物种中的关注有限。在鱼类中同源分子的发现取得了进展。在这里，我们报告了重组金鱼 M17 的促炎特性的特征。重组金鱼 M17 增强了吞噬作用，启动了活性氧中间体的产生，并且对巨噬细胞具有趋化性。用 LPS、热灭活和活菌嗜水气单胞菌处理金鱼巨噬细胞，导致 M17 mRNA 水平升高。重组 M17（RgM17）在金鱼巨噬细胞中诱导剂量依赖性 IFNγ 和 IL-1β1 的产生。此外，rgM17 处理巨噬细胞导致对髓样祖细胞分化为单核细胞/巨噬细胞（Runx1 和 GATA2）的重要转录因子的上调。我们的结果表明，金鱼 M17 是金鱼祖细胞增殖和分化所必需的炎症细胞因子。
