Hribar-Lee Barbara
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia.
J Mol Liq. 2022 Nov 15;366. doi: 10.1016/j.molliq.2022.120349. Epub 2022 Sep 14.
The aggregation propensity of monoclonal antibodies can be modified by adding different cosolutes into the solution. A simple coarse-grained model in the combination with the thermodynamic perturbation theory was used to predict cluster distribution and viscosity of the solutions of IgG4 monoclonal anibody in the presence of L-Arginine Hydrochloride. The data were analysed using binding polynomial to describe the binding of cosolute (Arginine) to the antibody molecule. The results show that by binding to the antibody molecule the cosolute occupies some of the binding sites of the antibody, and in this way reduces the amount of binding sites available to other antibody molecules. The aggregation propensity of the antibody molecules is therefore reduced.
通过向溶液中添加不同的共溶质，可以改变单克隆抗体的聚集倾向。结合热力学微扰理论的简单粗粒度模型被用于预测在存在盐酸L-精氨酸的情况下IgG4单克隆抗体溶液的聚集体分布和粘度。使用结合多项式分析数据以描述共溶质（精氨酸）与抗体分子的结合。结果表明，通过与抗体分子结合，共溶质占据了抗体的一些结合位点，从而减少了其他抗体分子可用的结合位点数。因此，抗体分子的聚集倾向降低。
