尼日利亚传统乳制品中抗生素耐药肠细菌的发生情况。

The occurrence of antibiotic-resistant enteric bacteria in Selected Nigerian traditional dairy products.

机构信息

Department of Microbiology, Faculty of Science, University of Lagos, Lagos, Nigeria.

出版信息

Afr Health Sci. 2022 Dec;22(4):619-626. doi: 10.4314/ahs.v22i4.67.

DOI:10.4314/ahs.v22i4.67

PMID:37092105

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10117513/

Abstract

BACKGROUND

and are popular dairy products in Nigeria, rich in nutrients, highly exposed to microbial contaminants during processing and sale and support microbial growth.

OBJECTIVES

To investigate occurrence and antibiotic resistance pattern of enteric bacterial pathogens in dairy products.

METHODS

Dairy products were serially diluted and cultured on Eosin Methylene Blue agar, agar, McConkey agar and nutrient agar at 37°C for 24 h. Characterisation and identification of isolates with API 20E kit (Biomereux, France). Antibiotic susceptibility was with agar disc diffusion. Polyvalent O and H antisera for serotyping.

RESULTS

serovar Enteritidis, serovar Typhimurium, serovar Typhi, and , were identified. Dominant enteric bacterium detected was followed by spp. Serratia marcescens was the least occurring. The isolates were most resistant to trimethoprim-sulfamethoxazole (96.7%), amoxicillin (83.3%), augmentin (83.3%), chloramphenicol (66.7%), streptomycin (50%). They were resistant to ≥ 4 (multiple) antibiotics, 8, spp. 7, 6 and spp. and spp. 4 each.

CONCLUSION

The presence of enteric bacterial pathogens in and and their resistance to multiple antibiotics was reported in this study.

摘要

背景

在尼日利亚，和是受欢迎的乳制品，它们富含营养物质，在加工和销售过程中极易受到微生物污染，并为微生物生长提供了支持。

目的

调查乳制品中肠道细菌病原体的发生情况和抗生素耐药模式。

方法

乳制品经过连续稀释后，在伊红美蓝琼脂、琼脂、麦康凯琼脂和营养琼脂上于 37°C 下培养 24 小时。采用 API 20E 试剂盒（法国生物梅里埃）对分离株进行特征和鉴定。采用琼脂扩散法进行抗生素敏感性试验。采用多价 O 和 H 抗血清进行血清型鉴定。

结果

鉴定出肠炎沙门氏菌血清型、鼠伤寒沙门氏菌血清型、伤寒沙门氏菌血清型、和。检测到的主要肠道细菌是，其次是 spp.。最不常见的是粘质沙雷氏菌。分离株对甲氧苄啶-磺胺甲噁唑（96.7%）、阿莫西林（83.3%）、氨苄西林（83.3%）、氯霉素（66.7%）、链霉素（50%）的耐药性最强。它们对≥4 种（多种）抗生素耐药， 8 株 spp.、7 株、6 株 spp.和 4 株 spp.耐药。