草酸钙结石形成者的激素和分子特征预测其发生和复发。
Hormonal and molecular characterization of calcium oxalate stone formers predicting occurrence and recurrence.
机构信息
Department of Urology, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Faculty of Science, Mansoura University, Mansoura, Egypt.
出版信息
Urolithiasis. 2023 Apr 24;51(1):76. doi: 10.1007/s00240-023-01440-8.
The purpose of the study is to investigate the role of sex hormones, androgen receptors (ARs) and miRNA/CSF-1 in occurrence and recurrence of calcium oxalate (CaOx) renal urolithiasis. In this prospective study, 74 patients with CaOx stones; stone formers group (SFG) and 40 healthy subjects; control group were compared. SFG includes both de novo and recurrent cases. Steroid sex hormone plasma assay including testosterone, free testosterone, dihydrotestosterone, estradiol, and sex hormone binding globulin was analyzed. ARs, miRNA-185-5p and CSF-1 expression were compared between the groups. SFG showed significant higher ARs and miRNA-185-5p expression (3.7 ± 1.3, 1.8 ± 0.4, respectively) than control group (1 ± 0.08 and 1 ± 0.07, respectively) (p < 0.05). However, CSF-1 expression was significantly lower in stone formers than control group (0.4 ± 0.19 vs 1 ± 0.1, respectively) (p < 0.05). No differences were detected between de novo and recurrent SFG regarding sex hormones, AR, miRNA or CSF-1 expression. Our data suggest the important role of AR, miRNA and CSF-1 signaling in human nephrolithiasis pathogenesis.
本研究旨在探讨性激素、雄激素受体 (ARs) 和 miRNA/CSF-1 在草酸钙 (CaOx) 肾尿石症的发生和复发中的作用。在这项前瞻性研究中，比较了 74 例 CaOx 结石患者（结石形成组 [SFG]）和 40 名健康受试者（对照组）。SFG 包括新发病例和复发病例。分析了甾体性激素血浆测定，包括睾酮、游离睾酮、二氢睾酮、雌二醇和性激素结合球蛋白。比较了各组之间的 ARs、miRNA-185-5p 和 CSF-1 表达。SFG 的 ARs 和 miRNA-185-5p 表达（分别为 3.7±1.3 和 1.8±0.4）明显高于对照组（分别为 1±0.08 和 1±0.07）（p<0.05）。然而，结石形成者的 CSF-1 表达明显低于对照组（分别为 0.4±0.19 和 1±0.1）（p<0.05）。新发病例和复发病例 SFG 之间的性激素、AR、miRNA 或 CSF-1 表达无差异。我们的数据表明，AR、miRNA 和 CSF-1 信号在人类肾结石发病机制中具有重要作用。
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