Institute of Genetic and Regenerative Biology, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
Hainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Sanya, 572025, China.
Commun Biol. 2023 Apr 25;6(1):457. doi: 10.1038/s42003-023-04835-w.
In rice (Oryza sativa) tissue culture, callus can be induced from the scutellum in embryo or from the vasculature of non-embryonic organs such as leaves, nodes, or roots. Here we show that the auxin signaling pathway triggers cell division in the epidermis of the scutellum to form an embryo-like structure, which leads to callus formation. Our transcriptome data show that embryo-, stem cell-, and auxin-related genes are upregulated during scutellum-derived callus initiation. Among those genes, the embryo-specific gene OsLEC1 is activated by auxin and involved in scutellum-derived callus initiation. However, OsLEC1 is not required for vasculature-derived callus initiation from roots. In addition, OsIAA11 and OsCRL1, which are involved in root development, are required for vasculature-derived callus formation but not for scutellum-derived callus formation. Overall, our data indicate that scutellum-derived callus initiation is regulated by an embryo-like development program, and this is different from vasculature-derived callus initiation which borrows a root development program.
在水稻（Oryza sativa）组织培养中，可以从胚的盾片中或从非胚器官（如叶片、节或根）的脉管系统中诱导愈伤组织。在这里，我们表明，生长素信号通路触发盾片表皮细胞分裂形成类似胚胎的结构，从而导致愈伤组织形成。我们的转录组数据表明，在盾片衍生的愈伤组织起始过程中，胚胎、干细胞和生长素相关基因上调。在这些基因中，胚胎特异性基因 OsLEC1 被生长素激活，并参与盾片衍生的愈伤组织起始。然而，OsLEC1 对于根脉管系统衍生的愈伤组织起始不是必需的。此外，参与根发育的 OsIAA11 和 OsCRL1 对于脉管系统衍生的愈伤组织形成是必需的，但对于盾片衍生的愈伤组织形成不是必需的。总体而言，我们的数据表明，盾片衍生的愈伤组织起始受类似胚胎发育程序的调控，这与借用根发育程序的脉管系统衍生的愈伤组织起始不同。
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