13-氧代-9Z,11E-十八碳二烯酸下调 c-Myc 并减弱乳腺癌细胞干性。
13-Oxo-9Z,11E-octadecadienoic Acid Down-regulates c-Myc and Attenuates Breast Cancer Cell Stemness.
机构信息
Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju, Republic of Korea.
Bio-Health Materials Core-Facility Center, Jeju National University, Jeju, Republic of Korea.
出版信息
In Vivo. 2023 May-Jun;37(3):1085-1092. doi: 10.21873/invivo.13183.
BACKGROUND/AIM: Breast cancer stem cells (BCSCs) are involved in the development of breast cancer and contribute to therapeutic resistance. This study aimed to investigate the anticancer stem cell (CSC) mechanism of 13-Oxo-9Z,11E-octadecadienoic acid (13-Oxo-ODE) as a potent CSC inhibitor in breast cancer.
MATERIALS AND METHODS
The effects of 13-Oxo-ODE on BCSCs were evaluated using a mammosphere formation assay, CD44/CD24 analysis, aldehyde dehydrogenase (ALDH) assay, apoptosis assay, quantitative real-time PCR, and western blotting.
RESULTS
We found that 13-Oxo-ODE suppressed cell proliferation, CSC formation, and mammosphere proliferation and increased apoptosis of BCSCs. Additionally, 13-Oxo-ODE reduced the subpopulation of CD44/CD24 cells and ALDH expression. Furthermore, 13-Oxo-ODE decreased c-myc gene expression. These results suggest that 13-Oxo-ODE has potential as a natural inhibitor targeting BCSCs through the degradation of c-Myc.
CONCLUSION
In summary, 13-Oxo-ODE induced CSC death possibly through reduced c-Myc expression, making it a promising natural inhibitor of BCSCs.
背景/目的：乳腺癌干细胞（BCSCs）参与乳腺癌的发生，并导致治疗耐药。本研究旨在探讨 13-氧代-9Z，11E-十八碳二烯酸（13-Oxo-ODE）作为一种有效的乳腺癌干细胞抑制剂的抗癌干细胞（CSC）机制。
材料和方法
采用类乳腺球体形成实验、CD44/CD24 分析、醛脱氢酶（ALDH）检测、细胞凋亡检测、实时定量 PCR 和 Western blot 等方法评价 13-Oxo-ODE 对 BCSCs 的作用。
结果
我们发现 13-Oxo-ODE 抑制了 BCSCs 的增殖、CSC 形成和乳腺球体增殖，并增加了 BCSCs 的凋亡。此外，13-Oxo-ODE 降低了 CD44/CD24 细胞和 ALDH 表达的亚群。此外，13-Oxo-ODE 降低了 c-myc 基因的表达。这些结果表明，13-Oxo-ODE 通过降解 c-Myc 可能具有作为 BCSCs 天然抑制剂的潜力。
结论
综上所述，13-Oxo-ODE 诱导 CSC 死亡可能是通过降低 c-Myc 表达实现的，使其成为一种有前途的 BCSCs 天然抑制剂。
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