系统鉴定百合基因家族及在花诱导和下胚轴伸长中的功能分析。

Systematical Characterization of the Gene Family in L. and the Functional Analysis of the in Flower Induction and Hypocotyl Elongation.

机构信息

College of Forestry, Hebei Agricultural University, Baoding 071000, China.

出版信息

Int J Mol Sci. 2023 Apr 14;24(8):7244. doi: 10.3390/ijms24087244.

DOI:10.3390/ijms24087244

PMID:37108407

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10138636/

Abstract

AT-hook motif nuclear localization (AHL) proteins play essential roles in various plant biological processes. Yet, a comprehensive understanding of transcription factors in walnut ( L.) is missing. In this study, 37 gene family members were first identified in the walnut genome. Based on the evolutionary analysis, genes were grouped into two clades, and their expansion may occur due to segmental duplication. The stress-responsive nature and driving of developmental activities of genes were revealed by -acting elements and transcriptomic data, respectively. Tissue-specific expression analysis showed that had a profound transcription in flower and shoot tip, in particular. Subcellular localization showed that JrAHL2 is anchored to the nucleus. Overexpression of in Arabidopsis adversely affected hypocotyl elongation and delayed flowering. Our study, for the first time, presented a detailed analysis of genes in walnut and provided theoretical knowledge for future genetic breeding programs.

摘要

AT-hook motif 核定位（AHL）蛋白在各种植物生物学过程中发挥着重要作用。然而，对于核桃（L.）中的转录因子还缺乏全面的了解。在这项研究中，首先在核桃基因组中鉴定了 37 个基因家族成员。基于进化分析，将基因分为两个分支，它们的扩张可能是由于片段重复造成的。通过顺式作用元件和转录组数据分别揭示了基因的应激响应性质和对发育活动的驱动。组织特异性表达分析表明，在花和茎尖中，有深刻的转录，特别是。亚细胞定位表明 JrAHL2 锚定在细胞核中。在拟南芥中过表达会对下胚轴伸长产生不利影响，并延迟开花。我们的研究首次对核桃中的基因进行了详细分析，为未来的遗传育种计划提供了理论知识。