Guangdong Laboratory for Lingnan Modern Agriculture, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2023 Apr 16;24(8):7346. doi: 10.3390/ijms24087346.
Some progress has been made in understanding the pathways related to rice heading, but their applications to breeding rice varieties adapted to grow in low-latitude areas (" to ") are limited. We edited eight adaptation-related genes via a lab-established CRISPR/Cas9 system in a variety, Shennong265 (SN265). All T plants and their progeny bearing random mutation permutations were planted in southern China and screened for changes in heading date. We found that the double mutant of 2 () and () (-), two () genes, showed significantly delayed heading under both short-day (SD) and long-day (LD) conditions in Guangzhou and manifested great yield increase under SD conditions. We further demonstrated that the heading-related - pathway was down-regulated in the - mutant lines. The editing of the genes and greatly improves the agronomic performance of rice in Southern China.
在理解与水稻抽穗相关的途径方面已经取得了一些进展,但将其应用于培育适应低纬度地区生长的水稻品种(“to”)的应用有限。我们通过实验室建立的 CRISPR/Cas9 系统编辑了一个品种 Shennong265(SN265)中的八个与适应相关的基因。所有携带随机突变的 T 植物及其后代都被种植在中国南方,并对抽穗期的变化进行了筛选。我们发现,两个 ()基因的双突变体 ()和 ()(-)在广州的短日(SD)和长日(LD)条件下表现出明显的抽穗延迟,并在 SD 条件下表现出产量的显著增加。我们进一步证明,-途径在 -突变体中下调。这两个基因的编辑极大地提高了南方水稻的农艺性能。