Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.
Beijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Int J Mol Sci. 2023 Apr 19;24(8):7529. doi: 10.3390/ijms24087529.
Ascorbic acid (AsA) is an antioxidant with significant functions in both plants and animals. Despite its importance, there has been limited research on the molecular basis of AsA production in the fruits of L. In this study, we used Illumina transcriptome sequencing (RNA-seq) technology to explore the candidate genes involved in AsA biosynthesis in L. A total of 8272 differentially expressed genes (DEGs) were identified by the comparative transcriptome analysis. Weighted gene co-expression network analysis identified two co-expressed modules related to the AsA content (purple and light-cyan modules), and eight interested DEGs related to AsA biosynthesis were selected according to gene annotations in the purple and light-cyan modules. Moreover, we found that the gene GDP-L-galactose phosphorylase (GGP) was related to AsA content, and silencing led to a reduction in the AsA content in fruit. These results demonstrated that is an important gene controlling AsA biosynthesis in the fruit of L. In addition, we developed capsanthin/capsorubin synthase as the reporter gene for visual analysis of gene function in mature fruit, enabling us to accurately select silenced tissues and analyze the results of silencing. The findings of this study provide the theoretical basis for future research to elucidate AsA biosynthesis in L.
抗坏血酸（AsA）是一种抗氧化剂，在植物和动物中都具有重要功能。尽管其重要性，但是对于 L. 果实中 AsA 生产的分子基础的研究有限。在这项研究中，我们使用 Illumina 转录组测序（RNA-seq）技术来探索与 L. 中 AsA 生物合成相关的候选基因。通过比较转录组分析，共鉴定出 8272 个差异表达基因（DEGs）。加权基因共表达网络分析鉴定出与 AsA 含量相关的两个共表达模块（紫色和浅青色模块），并根据紫色和浅青色模块中的基因注释选择了与 AsA 生物合成相关的八个感兴趣的 DEGs。此外，我们发现 GDP-L-半乳糖磷酸化酶（GGP）基因与 AsA 含量有关，沉默 会导致果实中 AsA 含量降低。这些结果表明 是控制 L. 果实中 AsA 生物合成的重要基因。此外，我们开发了辣椒素/辣椒红素合酶作为报告基因，用于成熟果实中基因功能的可视化分析，使我们能够准确选择沉默组织并分析沉默的结果。这项研究的结果为未来研究阐明 L. 中的 AsA 生物合成提供了理论基础。
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