Suh Seung-Man, Kim Eiseul, Kim Mi-Ju, Yang Seung-Min, Kim Hae-Yeong
Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104 South Korea.
Food Sci Biotechnol. 2023 Jan 10;32(7):979-985. doi: 10.1007/s10068-022-01231-8. eCollection 2023 Jun.
Centipedes contain pharmacologically active compounds used as important medicinal material. However, the poisons produced by centipedes can cause human diseases; therefore, its use as a food ingredient is prohibited. This is the first report to develop a real-time PCR method for detection of centipedes. The primer and probe targeting the mitochondrial cytochrome c oxidase subunit 1 (COI) gene were newly designed. The specificity was verified using ten species and was confirmed to amplify only the centipede species. The real-time PCR method exhibited good linearity with a high-determination coefficient ( = 0.999) and a detection limit was 0.001 ng. The performance of our method was also verified using five real-time PCR platforms under Universal and Fast PCR conditions. Finally, its applicability to processed food was evaluated using binary insect mixtures, and at least 0.1% of centipedes was detected. Therefore, our method can specifically and sensitively detect centipedes in food, contributing to food safety.
蜈蚣含有用作重要药材的药理活性化合物。然而，蜈蚣产生的毒素可导致人类疾病；因此，禁止将其用作食品成分。这是第一份开发用于检测蜈蚣的实时PCR方法的报告。新设计了靶向线粒体细胞色素c氧化酶亚基1（COI）基因的引物和探针。使用十种物种验证了特异性，并确认仅扩增蜈蚣物种。实时PCR方法表现出良好的线性，具有高决定系数（ = 0.999），检测限为0.001 ng。在通用和快速PCR条件下，还使用五个实时PCR平台验证了我们方法的性能。最后，使用二元昆虫混合物评估了其在加工食品中的适用性，并检测到至少0.1%的蜈蚣。因此，我们的方法可以特异性和灵敏地检测食品中的蜈蚣，有助于食品安全。
