Department of Biology, Faculty of Education for Pure Science, University of Kirkuk, Kirkuk, Iraq.
Arch Razi Inst. 2022 Oct 31;77(5):1521-1525. doi: 10.22092/ARI.2022.357753.2095. eCollection 2022 Oct.
Klebsiella spp. are gram-negative bacteria that are considered serious public health problems causing urinary tract infections, bloodstream infections, pneumonia infections, and soft tissue infections. This study was designed to investigate the prevalence of () among clinical samples and determine their resistance against various antimicrobial medicines with molecular identification of by polymerase chain reaction (PCR) technique using a specific sequence of pehX gene. A total of 250 clinical samples including throat, wound, and vaginal swabs were obtained. Participants were of both genders and different ages. The samples were streaked on the blood and MacConkey agars. Antibiotic sensitivity test was made by modified Kirby-Bauer disc diffusion technique. Molecular identification of was performed for all isolates. Out of 250 clinical samples, was reported in 32 (12.8%) cases. The highest prevalence was observed in 18(18%) cases of throat swabs, 16 (16%) cases of wound swabs, and 6 (6%) cases of vagina swabs. By the way, female cases were more affected 22 (14.5%) with than male cases 10 (10.10%). Infected participants aged 15-40 years were more affected with (23, 12.73%) compared to patients aged 41-65 years (9, 9.67%). The highest resistance pattern of was 100% against Augmentin, Ampicillin, Cephalothin, Piperacillin, and Rifampin on one hand, and 62.50%, 59.37%, 53.12%, 53.12%, and 50% against Ceftazidime, Cefixime, Cefotaxime, Trimethoprim, and Aztreonam on the other hand, respectively. The highest sensitivity was observed against Amikacin and Imipenem (9.37%) and it was 21.87%, 21.87%, 25%, 25%, 28.12%, 28.12%, and 28.12% against Meropenem, Chloramphenicol, Nalidixic acid, Ciprofloxacin, Tobramycin, Gentamicin, and Doxycycline, respectively. Through molecular identification of , all isolates showed a PCR product with 344-bp specific primer (pehX) that performed the .
产酸克雷伯菌属是革兰氏阴性菌，被认为是严重的公共卫生问题，可导致尿路感染、血流感染、肺炎感染和软组织感染。本研究旨在调查临床样本中产酸克雷伯菌属的流行情况，并通过聚合酶链反应（PCR）技术用 pehX 基因的特定序列对其进行分子鉴定，以确定其对各种抗菌药物的耐药性。共采集了 250 份临床样本，包括咽喉、伤口和阴道拭子。参与者为不同性别和不同年龄的人。将样本划线接种于血琼脂和 MacConkey 琼脂平板上。采用改良 Kirby-Bauer 纸片扩散法进行抗生素敏感性试验。对所有分离株进行 的分子鉴定。在 250 份临床样本中，报告了 32 例（12.8%）产酸克雷伯菌属感染。咽喉拭子中检出率最高，为 18 例（18%），伤口拭子中检出率为 16 例（16%），阴道拭子中检出率为 6 例（6%）。此外，女性病例（22 例，14.5%）比男性病例（10 例，10.10%）更易感染。15-40 岁的感染者较 41-65 岁的感染者更易感染产酸克雷伯菌属（23 例，12.73%）。产酸克雷伯菌属的最高耐药模式为对氨芐西林、氨苄西林、头孢噻吩、哌拉西林和利福平均表现为 100%耐药，对头孢他啶、头孢克肟、头孢噻肟、甲氧苄啶和氨曲南的耐药率分别为 62.50%、59.37%、53.12%、53.12%和 50%。对阿米卡星和亚胺培南的敏感性最高（9.37%），对美罗培南、氯霉素、萘啶酸、环丙沙星、妥布霉素、庆大霉素和强力霉素的敏感性分别为 21.87%、21.87%、25%、25%、28.12%、28.12%和 28.12%。通过 的分子鉴定，所有分离株均显示出 344-bp 特异性引物（pehX）的 PCR 产物，完成了 鉴定。
